染色法

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-（1-7）, positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞，鉴定后传代培养，取4～7代细胞，应用不同浓度ang-（1-7）干预，并与血管紧张素-ii(ang-ii，浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法，确定肺动脉平滑肌细胞的生长趋势变化。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

METHODS: Eighty guinea pigs were randomized into control group, model group, salbutamol treated group and Maxin liquid treated group. Asthma model s were established by ovalhumin. The changes of eosinophile in bronchoalveolar lavage fluid, thromboxane B2 and 6-Keto-prostaglandin F1a in blood, the airway resistance and the pulmonary compliance were observed by Pap staining, radio-immunity , and pulmonary function test instrument of MIL type specially for experimental animal.

实验设正常对照组、模型对照组、复方麻辛组、沙丁胺醇组，选用豚鼠共80只，采用清蛋白造模，并运用巴氏染色法、放射免疫法、MIL型实验动物肺功能测量仪，分别检测肺泡灌洗液中嗜酸粒细胞数、血浆凝血恶烷(TXB2)、6-酮－前列腺素(6-K-PGF1a)以及清蛋白激发后气道阻力和肺顺应性。

With the help of Axioplan 2 imaging system, we quantified the change of expression of ICAM-1, MUC5AC, goblet cell and NF-κB. Analyze the results by SAS method. Results (1) Grouped by index of lung function, the expression of ICAM-1 at the level of gene and protein had increased significantly in the epithelial cells of patients with COPD, compared with group of normal lung function.

取病灶远端的正常叶段支气管组织制备石蜡切片，原位杂交检测气道上皮中ICAM-1的mRNA的表达量；免疫组化检测气道上皮中ICAM-1和MUC5AC的蛋白表达变化；AB/PAS特殊染色法检测患者气道上皮细胞中杯状细胞的含量；PCR扩增法检测原代培养的气道上皮中MUC5AC的基因表达量和NF-κB的变化。

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况，用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide or caspase-3 inhibitor (DEVD-CHO, 100 μmol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and IκΒ-α were detected by Western blotting methods.

用二甲基亚砜对照、100μmol/L caspase-3抑制剂预处理乳腺癌Bcap37细胞3h后，加入不同浓度的长春碱，以MTT法检测肿瘤细胞增殖能力，以细胞DNA片段分析及PI染色法检测肿瘤细胞凋亡，以蛋白免疫印迹法检测pro-caspase-3和IκΒ-α蛋白的变化。

The tinction and morphology of MGC-803 cells treated with NGF at different final concentrations of 25,50 and 75mg/ L for 24h were observed by acridine orange/ethidium bromide double staining.

4采用AO/EB双重染色法，NGF浓度分别为25，50，75mg/L，观察不同浓度NGF作用后MGC-803细胞的染色及形态变化。

P19 differentiation was induced by retinoic acid, and P19 cells cultured on gelatin were used as control. Differentiated cells were observed with microscope. TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation. Survival cells were detected by AlamarBlueTM method.

视黄酸(retinoic acid，RA)诱导P19细胞分化，培养于明胶包被培养板上的P19细胞为对照组，普通光学显微镜观察其神经分化情况，TURNEL法和DNA片段化分析法检测细胞凋亡情况，alamarBlueTM染色法检测细胞存活情况。

The asbestos fiber becomes coated with iron and calcium, which is why it is often referred to as a "ferruginous body" as seen here with an iron stain.

石棉纤维被铁和钙包裹，这就是采用铁染色法染色发现含铁体的原因。

Six and twelve boors after sepsis, leptin levels in liver homogenate were detected by radioimmunoassay serum alanine aminotransferase and 4 enzymes in liver homogenate. myeloperoxidase, glutathin-S-transferase, xanthine oxidase and superoxide dismutase, all related with synthesis of free radicals, detoxication and purine metabolism, were detected by 96 well spectrophotometry. H-E staining was used to examine the histopathologic changes of liver.

于脓毒症后6 h和12 h，采用放射免疫法测量肝组织匀浆液中Leptin水平，采用96孔分光光度法检测血清丙氨酸氨基转移酶及髓过氧化物酶、谷胱甘肽－S转移酶、黄嘌呤氧化酶、超氧化物歧化酶等4种与自由基合成、解毒和嘌呤生成代谢相关的酶的水平，同时以H-E染色法观察肝组织病理学改变。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐