染色法

The second part of the study examined the distribution of type Ⅰ and Ⅲ collagens, fibronectin and laminin in murine liver using protein A gold silver tealnique.

研究的第二部分建立了光镜水平的葡萄球菌A蛋白金银染色法并将其运用于对肝脏基质抗原FN和LN的免疫细胞化学研究，获得了优于免疫荧光法和免疫酶法的结果。

It was the first time among domestic researches that small diameter log of Populus tomentosa dyed with stumpage dyeing method was studied.

本论文首次在国内用立木染色法对毛白杨小径材进行染色。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物，用低维生素E且添加鱼油日粮饲喂雏鸡，复制脑软化症动物模型，采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标，并对患雏病理组织进行了显微及超微结构观察。

The pulmonary tissues taken from autopsies performed in 1988～2001 including 19 AFE cases and 3 babies died of amniotic fluid aspiratory pneumonia,10 women in labor died of other causes were sectioned again and stained by APM method and HE method.

采用APM染色法对 1988年至 2 0 0 1年确诊为AFE的 19例病例和羊水吸入性肺炎的胎儿 3例、其它死因的 10例产妇重新进行染色和组织学检查，对其检验结果进行比较分析。

Methods Semen analyses were carried out according to the World Health Organization manual for 47 subfertile men,film preparation before and after centrifugate washing,then use HE dyeing,and assess the deformity ratio of sperm headbodytail and SDITZI.

47例人精液标本进行常规检测密度及活力后，分别在离心前后涂片，应用快速苏木素-伊红染色法进行染色，检测精子头部、体部、尾部畸形率。对比精子畸形指数和畸形精子指数是否受到离心洗涤的影响。

Methods Semen analyses were carried out according to the World Health Organization manual for 47 subfertile men,film preparation before and after centrifugate washing,then use HE dyeing,and assess the deformity ratio of sperm head,body,tail and SDI,TZI.

47例人精液标本进行常规检测密度及活力后，分别在离心前后涂片，应用快速苏木素-伊红染色法进行染色，检测精子头部、体部、尾部畸形率。对比精子畸形指数和畸形精子指数是否受到离心洗涤的影响。

Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining.

mg/L的LPS作用于细胞，采用MTT染色法检测LPS对细胞增殖的影响；采用流式细胞术检测细胞周期及凋亡；采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。

The proliferation of NPCs detected by 5-bromodeoxyuridine incorporation and LacZ staining was restricted to the ependymal zone of the central canal.

这种增殖可以被5-溴脱氧尿苷混合物检出。中央管的室管膜带可以被LacZ染色法染色。

Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR.

进一步对SOCS-3基因沉默后的K562细胞进行了诱导分化，并采用联苯胺染色法检测K562细胞向红系分化比例变化，免疫荧光染色检测细胞表面抗原的变化，RT-PCR 检测造血相关基因的变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。