染色法

Methods Wistar middle cerebral artery occlusion reperfusion rat model was established by thread ligation. The infarction focus formation was observed by TTC staining. AIF expression in ischemia region of cortex of parietal lobe and nerve cell apoptosis degree in sham operation group, MCAO 90 min and reperfusion 0, 3, 6, 12, 24, 48 and 72 h were observed by TUNEL and immunohistochemistry technique.

应用线栓法建立Wistar大鼠大脑中动脉阻塞再灌注模型，TTC染色观察梗死灶的形成，应用原位末端标记和免疫组化技术分别观察大鼠假手术组、MCAO 90 min再灌注0、3、6、12、24、48、72 h时额顶叶皮层缺血脑区AIF表达及神经细胞凋亡程度的变化。

The germinal vesicle can beisolated successfully in such a physical method and it can undergo chromatincondensation in vitro just as the oocyte does in vivo.

用直接取核法可成功分离出游离的生发泡，且它们在体外培养条件下可以和体内一样发生染色质凝集现象。

DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown.

采用直接取核法获取卵母细胞生发泡，用同步化的HeLa细胞裂解液构建非细胞体系，将游离的生发泡培养在裂解液中，并加入荧光染料Hoechest 33342，观察染色质的变化情况。

Cooked using the process of German companies BASF professional fur cooked reagents, using the most advanced production of chrome rubbing the skin and hair of a centralized processing, the main process are: skin pretreatment ---- 24小时Baptist acid -- Paper plates - rubbing the oil to heat the system ---- skim softening --------- forming fat -- stain-kneading fine - hot - such as dozens of fine cut Road, skin color into the finished product production Section, according to a fineness of wool ratio, and then after cutting, stitching, shape, and quality inspection procedures can only be a few dozen packaging factory.

熟制过程使用德国BASF公司的专业裘皮熟制试剂，采用国际最先进的铬揉生产法，对皮和毛进行集中统一化的处理，主要工艺有：原皮预处理----24小时浸酸---皮板去油---揉制----高温脱脂---成型---软化---加脂—复揉---染色---精烫--精剪等数十道，成品色皮进入成品生产工段后，还要根据毛质细度进行配比，再经过裁剪，缝合，成型，及质量检验等几十道工序才可以包装出厂。

In this paper, the diffusion and permeability parameter of dyestuffs permeating terylene fabric under different conditions in supercritical dyeing system were investigated. The results indicated that the parameter decreased with temperature rise, and increased with pressures increase.

在超临界CO2染色条件下采用&卷层法&对分散染料在涤纶织物中的扩散渗透进行了考察，结果表明：染料在涤纶织物中的扩散渗透参数随着温度的升高而减小，随着压力的增高而增大。

Of, relating to, or being a bacterium that does not retain the violet stain used in Gram's method .

革兰氏阴性的属于，有关或作为用革兰氏法染色不保留紫色色素的细菌的

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。