染色法

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

In this paper the distribution of CGRP neurons and the relationship between CGRP nerve terminals and catecholaminergic neurons in the nucleus tractus solitarii of the rat was studied at light microscopic and electron microscopic levels, using double-immunostaining method and immunoelectronmicroscopic staining techniques.

用双重免疫染色包埋前免疫电镜法，在光镜和电镜水平观察了大鼠孤束核内降钙素基因相关肽神经元的分布及其神经末梢与儿茶酚胺能神经元之间的关系。

Methods We observedhe distribution of aFGF,bFGF and their change after SCI with immunohistochemical staning, and staining was quantitated by imagine analysis technique.

采用 S-P法免疫组化染色方法，观察aFGF、bFGF在脊髓中的分布，以图像分析技术对其在SCI后的变化进行定量分析。

Methods: In accordance with the standardization of qualitycontrolHE and immunohistochemial stainings of puncture liver tissues were performed, and the results were.

按照质控标准化对298例肝脏穿刺组织行HE制片和免疫组化染色，盲法比较标准化前后病理诊断结果。

Methods H＆E staining, histochemical stainings and immunohistochemical stainings were used.

用普通形态学方法结合组织化学PAS法及免疫组织化学染色观察6例女性腹膜原发癌的形态特点。

Within 24 hours of the injection, IC was primarily distributed in blood vessels and sinusoids.

免疫组织化学ABC法染色发现，不溶性IC注射后24小时，主要见于血管系统如肝血窦和肾小球毛细血管之中。

The lumbar spinal cord in normal rat was immunohistochemically stained with ABC method using HAP1B antibody. Under light microscope, it was observed that HAP1 immunoreactants is mainly distributed in the pericaryon and neuropil in superficial lamella of the gray nucleus with little or no expression in other layers of the spinal horn. The substantia alba show no HAP1B immunoreactants.

对正常大鼠腰段脊髓进行HAP1B的ABC法免疫组织化学染色，光镜下观察显示，HAP1免疫反应产物主要分布在脊髓灰质背角浅层神经元胞体和神经毡内，灰质其他各层无或极少HAP1B表达，白质内未见明显HAP1B免疫反应产物。

The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor-PCR, PCR, smear and culture methods, and the results were compared.

采用AmpliSensor-PCR对784例结核病患者及160例肺癌患者的标本进行检测，并与PCR(凝胶电泳后，经溴化乙锭染色)、涂片、培养等法比较。

The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor-PCR, PCR, smear and culture methods, and the results were compared.

采用AmpliSensor-PCR对784例结核病患者及160例肺癌患者的标本进行检测传染病，并与PCR(凝胶电泳后，经溴化乙锭染色)、涂片、培养等法比较。

METHODS The inhibitory effect of nobiletin on proliferation of SMMC-7721 cells was assessed by MTT analysis and growth curve assay. Morphological changes under microscope were observed by Giemsa staining. Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of KM mice. Different dosages of nobiletin 125, 250, 500mgkg^(-1 were injected by intragastric gavage everyday.

不同浓度的川陈皮素作用于人肝癌细胞SMMC-7721细胞，用MTT法、细胞生长曲线实验研究其对SMMC-7721细胞的生长抑制作用，Giemsa染色观察细胞形态变化，初步研究其体外抑瘤作用；建立小鼠H22肝癌移植性实体瘤模型，研究川陈皮素对肝癌的体内抑制作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。