染色法

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

Through discussion on the dosage of assistant, curing temperature and time, the optimal process of padding was obtained, polyurethane-modified silicon emulsion 40 g/L of dosage, curing 2 min at 120℃. The results showed that polyurethane-modified silicon rubbing fastness improver could evidently enhance the dry and wet rubbing fastnesses of deep color fabrics. In addition, it could endow fabric with softness and smoothness.

通过对助剂用量、焙烘温度、焙烘时间的探讨，确定了浸轧法处理染色织物的最佳工艺条件：聚氨酯改性有机硅高分子乳液用量40g/L、120℃焙烘2 min经聚氨酯改性有机硅湿摩擦牢度提升剂处理的深浓色织物，其干、湿摩擦牢度得到了明显的提高，而且还能赋予织物柔软、滑爽的手感。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素（bleomycin，BLM）复制大鼠肺纤维化模型，并分离培养肺成纤维细胞，用阿托伐他汀对其进行干预，分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组，用倒置纤维镜观察细胞的形态结构，MTT计数法观察各组细胞的增殖情况，免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ，α-SMA)的表达情况。

Twelve L5 left dorsal root ganglions on each time point were harvested from sham group on the 7th day after operation and from model group on the 7th,14th and 21st day after operation and then were tested histologically by Hematoxylin-Eosin staining and the proportion of Cycloxygenase-2（COX-2） and p-p38MAPK positive expression cell were tested by immunohistochemistry method either.

测量各组大鼠术前至术后21d时左后肢50％机械性撤足阈值（50%PWT）以测定机械痛敏的变化，假手术组术后7d及模型组术后7d、14d、21d取左L5 DRG分别进行HE染色观察组织学变化和免疫组化法测定环氧化酶-2（COX-2）与p-p38MAPK的阳性细胞比率。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

Objective To study the effects of heat-killed Penicillium marneffei on the expressions of toll-like receptor-4 (TLR-4),toll-like receptor-2 (TLR-2) and dendritic cell associated C-type lectin-1 (Dectin-1)and the production of the proinflammatory cytokine tumor necrosis factor-α.

办法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

Results The signal intensity of infracted tissue on DWI images was markedly higher than that of normal brain tissue at one hour after stroke, and becaming higher and higher at the following three scan time.

测量ADC值、T2信号强度，根据CD34免疫组织化学染色结果计数毛细血管数，方差分析法分析不同时间点结果的差异，并进行相关性分析。

Each group was treated by intraperitoneal injection once every two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum, expression of ED-1 by Kupffer cell was analyzed by immunohistochemistry.

采用原子吸收分光光度法测定铝元素在肝组织中含量的变化；免疫组织化学染色观察肝组织中枯否细胞的活化；RT-PCR检测凋亡相关基因Bax及Bcl-2 mRNA表达的改变。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

Pulp reactions were obvious during 3 days after lased. Alkaline phosphatase examination: After fixed, decalcified and selected, all experimented teeth were stained with the Ca-Co method in order to examine the alkaline phosphatase activity in dental pulp cells.

碱性磷酸酶的检测：实验牙在丙酮中固定1 d，再置入复合酸中脱钙，冰冻切片后，进行钙-钴法染色以显示牙髓细胞中碱性磷酸酶活性，根据米德冷图象分析系统测定切片的灰度值，进行统计学分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。