染色法

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

Using special MSB staining method, early thrombi were presented in the necrotic model.

成功地应用显示血栓的特殊染色MSB法观察到该模型早期骨内血栓的存在。

In the 56 , 22 were in sinus ventriculi, 6 in corpus ventriculi and 28 in fundus and cardia ventriculi.Tumoer size that was smaller than 5cm were 34 cases,while that was bigger than 5cm were 22 cases.

所有标本常规经10％福尔马林固定，石蜡包埋，定向切片；分别采用NF-κBp65鼠单克隆抗体和uPA兔多克隆抗体，以S-P法进行免疫组织化学染色，观察其在各组织标本中的表达。

Hontonpur GWW is a high efficient dispersing agent, which can make the chemical yarn and its blends cleaning and dyeing in one bath in acid condition.

Hontonpur GWW 是一种高效强分散性助剂，它使化纤纱线及其混纺织物在酸性条件下完成一浴法工艺的净洗和染色。

The optimal usage of carrier was only 3%, the dye pickup, building up properties and migrating properties of BIP carrier to disperse dyes was superior to sweet birch oil.

载体BIP对分散染料的上染率、提升性、移染性均优于冬青油；染色后织物的表观深度和颜色特征与高温高压法相当。

Methods: The ratios of Th1/Th2 cells in the mouse with immunological liver injure were detected by flow cytometry with two color intracellular cytokine staining; the changes in substance P, vasoactive intestinal peptide in the pituitary gland and peripheral blood were detemined by radio-immunoassary.

应用尾静脉注射卡介苗和脂多糖建立免疫性肝损伤小鼠模型，分离小鼠淋巴细胞，经佛波醇乙酯、离子霉素、莫能菌素刺激培养6h后，收获细胞，利用细胞内细胞因子染色流式细胞术检测Th1/Th2比例：采用放免法检测血浆和脑垂体中SP、VIP含量。

Methods Blood vessel wall pathological and the serum lipid changes,the expressions of typeⅠcollagen, NF κB, typeⅠcollagen mRNA and NF κB mRNA levels were observed in dietary induced atherosclerosis rabbit model by morphology study, immunohistochemistry, in situ hybridization technique and color image analyzer.

采用HE、免疫组化及原位杂交法染色，光镜观察AS病变，并应用CMIAS真彩色医学图像分析系统分析；检测40只食饵性AS新西兰兔血脂成分的改变及血管壁的病理变化、Ⅰ型胶原mRNA与其蛋白、NF κBmRNA与其蛋白的表达情况。

Establishing the atherosclerotic model of rabbits by feeding them with cholesterol, we measured their body weight, TC, TG and LDL. Drawing the materials from rabbit aortic wall and embedding them with paraffin to make into section preparation, we detected type Ⅰ collagen, NF-κB, VCAM-1, CD36, type Ⅰ collagen mRNA and NF-κB mRNA with immunohistochemistry and in situ hybridization technique and by means of color image analyzer .

通过高脂饲料建立兔AS模型，测定体重及血脂成分的改变；取其主动脉壁，石蜡包埋制成切片，采用HE、免疫组化及原位杂交法染色，光镜观察AS病变，并应用CMIAS真彩色医学图像分析系统，检测Ⅰ型胶原mRNA及其蛋白、NF-κBmRNA及其蛋白、VCAM-1及CD36蛋白表达定量A值，与对照组进行比较。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

The effects of pure knitting wool dyeing and color setting in the double dye vat are studied.

研究了纯羊毛线染深色时二浴法染色固色的效果。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。