染色法

Methods:After diagnosis of varioliform gastritis was made by gastroscopy,biopsy was taken,regular pathological examination was made.

对胃镜诊断的痘疹性胃炎，取活检，常规病理检查，用快速尿素酶法及GIEMSA染色检测幽门螺旋杆菌。

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色，显微镜下进行观察并拍照，对Fos免疫阳性细胞进行形态与计数分析。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量，结果表明，虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin，白色闭壳肌中未检测到类胡萝卜素，其中肝胰腺除上述两种类胡萝卜素外，还含有其他多种未鉴定的类胡萝卜素；在各组织中，雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高；橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝；雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察，并未观察到异常现象；超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌，在橘红色闭壳肌中有三种肌纤维，其中疏松型肌纤维为橘红色闭壳肌特有。

Then animals were killed by gas embolism, aorta was separated from the beginning of aortic arch to abdominal aorta crotch. At the end of experiment, the aortas were prepared for measuration of the concentration of ceramide and cholesterol. The pathological changes and plaque area were measured.

第十周实验结束时，用气栓法处死动物，将主动脉全部取下，沿正中线剪开，摄像，同时作主动脉斑块面积分析及HE染色等主动脉病理学检测，并取血管组织用HPTLC测量血管胆固醇的含量。

After 8 weeks,PDGF-B measured with reverse transcription polymerise chain reaction and pathologic alter were observed with HE staining.

高脂喂养6周后以球囊拉伤方法建立兔颈总动脉内皮损伤模型。8周时处死动物，以逆转录聚合酶链式反应法检测动脉损伤处PDGF-B mRNA表达，HE染色观察颈动脉组织病理形态学改变。

Results HE staining indicated that the elastic fiber of aneurysmal wall, which plays a crucial role in the intact and stability of artery wall, was obviously destroyed when comparted with that of normal artery wall.

天坛医院2006年10月至2007年9月术中随机取得12例颅内动脉瘤，以同期5例脑肿瘤患者的颞浅动脉为对照，通过HE染色及Westerblot蛋白分析法进行研究。

Methods The expression of ERK1/2, ER and PR in 30 endometrial carcinoma specimens was detected by immunohistochemical method. With the same method, the expression of ERK1/2 was detected in 20 normal endometria specimens and 13 hyperplastic endometria specimens. Semi-quantitative analysis of specimen staining was conducted.

用免疫组化方法检测30例子宫内膜癌石蜡标本中ERK1/2以及ER和PR的表达；同法检测20例正常子宫内膜、13例增生过长子宫内膜石蜡标本中ERK1/2的表达；对标本的染色情况做半定量分析。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

The spleens and heart were examined pathologically and/or immunohistochemically. Results: Compared with those of control group, inflammatory cells infiltration in the myocardium and calcification in the pericardiume in SVitC and LVitC groups were extenuated.

小鼠于第21天给予称重，麻醉后处死，并摘眼球取血，用Elisa法测定血清肿瘤坏死因子α水平；取心脏、脾脏和肾脏分别称重，得心脏、脾脏和肾脏与体重之比(C/W、S/W、R/W)；脾脏在肉眼观察后作病理切片和H-E染色。

When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.22%, the highest rate in all groups. After the morulae cultured from thawed embryos from the best straw group and OPS group were transferred, all groups have obtained offspring.

在25℃温度条件下细管法和OPS二步法玻璃化冷冻后的小鼠2-细胞胚胎，经培养发育至囊胚后，用H33342染色，在荧光显微镜下观察并进行细胞计数。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。