染色法

Methods The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree determination, cell cycling analysis, karyotype analysis and heterotransplantation.

采用原代组织块培养法对成骨肉瘤手术标本进行培养，对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶同工酶谱、细胞周期检查、核型分析、异种移植。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

Methods After FK506 administration at a dose of 1 mg/kg body weight intramuscularly for 21 days the pigs were sacrificed and the thymus were removed.

对5头猪按1 mg/kg肌肉注射FK506 21 d，然后取猪胸腺制成细胞悬液，双色法间接免疫荧光染色后，以流式细胞仪检测。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

Results The neoplasms were usually well circumscribed and were solid, often have a lobulated appearance.

方法对1例非特异性类固醇细胞瘤进行HE和免疫组化EliVision法染色，并复习文献。

Vascular endotheliocyte fluorescence staining and leukocyte fluorescence tracing method were applied, in combination with microscopic video system and computer imaging analyzing system, to observe in dynamic and quantitatively the influences of acupuncture on pia matral encephali microvascular morphology, density and blood flowing velocity at 3 hours, 6 hours and 24 hours after cerebral occlusion of meddle cerebral artery.morphology, density and blood flowing state at various time divisions observed directly microscopically and vascular endotheliocyte morphology and the state of brain tissue which near the microvascular after infusion with velocity in infarcted area at various time divisions measured in quantity in each group.microvascular endotheliocytes were colored worse and more fluorescent exudation appeared.

采用血管内皮细胞荧光染色及白细胞荧光示踪法，结合显微录像系统和计算机图像分析系统，动态定量地观察了针刺对大脑中动脉阻塞后3，6，24h软脑膜微血管形态、密度、血流速度的影响。主要观察指标：①从显微镜下直接观察各组大鼠不同时段软脑膜微血管的形态、密度、血流状况，以及注入荧光剂后血管内皮细胞形态和周围脑组织状况。②定量测量各组大鼠不同时段梗死区微血管密度、血流速度。结果：120只大鼠均进入结果分析。

The cells were identified by invert phase contrast microscopy and alkaline phosphatase staining and mineralized nodules staining.The activity distribution of osteoblasts was determined by microtitration during a culture cycle.

通过倒置相差显微镜观察，并用碱性磷酸酶和矿化结节染色鉴定，利用微量滴定法测定一个培养周期的成骨细胞活性分布，探讨一种经济、高效的原代成骨细胞培养方法。

Simultaneously 15 samples of normal nephric tissue were used as control.The sections from these samples were subjected to immunohistochemical analysis with antibodies directly against uPA and PAI-1 by LSAB method. In this study we adopt semiquantitative analysis to determined the expression of these molecules , and utilized the relevant data processing and statistical analysis to evaluate the correlation between their expression and the clinicopathological features of RCC.Results: 1. The positive rate of uPA and PAI-1 of uPA in RCC were 72%(36/50)and 56%(28/50), respectively.But in control group there was nearly

正常肾组织标本中几乎无阳性染色。2、uPA表达水平与患者年龄、性别、肿瘤病理分级无关(均P＞0.05)，与T分期、淋巴结转移有明显的相关性(r=0.684，P＜0.05；r=0.789，P＜0.05)，uPA高表达多见于T_(3-4)、有转移的RCC.3、PAI-1的表达水平与患者年龄、性别、肿瘤病理分级和T分期无关(P＞0.05)，与淋巴结转移有明显的相关性(r=0.800，P＜0.05)，PAI-1高表达多见有转移的RCC.4、对uPA和PAI-1行相关性检验，uPA在RCC中的表达与PAI-1的表达呈明显正相关(r=0.168，P＜0.05)。5、uPA和PAI-1的表达与淋巴结转移的关系以Logistic回归法进行分析，结果显示uPA与区域淋巴结转移密切相关，uPA是淋巴转移的危险因子(RR=2.718，95％CI=1.150～7.170，P=0.024)，PAI-1则不是淋巴转移的危险因子(RR=1.599，95％CI=0.624～4.102，P=0.328)。

Were comparativey studied using immunocytochemical tech niques-peroxidase antiperoxidasemethod.These positive immunoreactive systems were compared with the neuroanatomical data on neuropile organization and single nerve cell type in the bee's brain.

通过免疫细胞化学方法－PAP法，研究了中华峰和意大利峰脑和视叶中乙酰胆碱、5－羟色胺（5－HT）、γ－氨基丁酸免疫反应阳性神经元胞体和纤维的形态、染色强度、分布和各种神经丛之间的联系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。