染色法

The antitumor activity of platinum complex of ammine/amine (NH3/CH3NH2) with oxalate was determined by MTT and SRB methods in vitro. At the same time, the anticancer mechanism was studied by flow cytometry, Giemsa and Icp-Ms.

采用MTT和SRB法研究了混胺(NH3/CH3NH2)草酸根合铂混配配合物对人体癌细胞的增殖抑制作用，又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制。

The freezing slices of brain tissue were made, which different locations were collected from the brains of 12 catties. Then using the antiserum as the first antiboty and the monoclonal antibody 4C11 as the collator, the slices digested or undigested by protease K were dyed by ABC dyeing. The result was used to determined the location of PrP in cattle brain.

以12健康头黄牛脑组织为研究对象，采取脑组织的不同部位制成冰冻切片，以自制抗血清为一抗和单抗4C11为参照，应用ABC染色法对蛋白酶K处理的和未作处理的切片染色，检测牛脑组织中朊蛋白的分布。

The fry of black seabreamand giant sea perch with 4.0cm-8.5cm using the methods of dyeing, i.e., the fish immersed in the water solution containing comestible pigment, immersion in tetracycline, i.e., the fish immersed in the water solution containing tetracycline, and marking by spraying, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish, and double marking, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish after feed with tetracycline, and control group. The survival rates, mark retention rates and hydrology data were separately measured every three days for black sea bream and every two days for giant sea perch. The results were as follows.

本研究针对体长4.0cm-8.5cm的黑鲷及金目鲈的鱼苗采用染色法(将鱼只浸泡在含有食用色素的水溶液中)、四环霉素浸泡法(将鱼只浸泡在含有四环霉素的水溶液中)、喷枪法(利用高压喷枪将萤光色素打在鱼的表皮)以及双重标识法（四环霉素投餵法加上喷枪法）与一组空白组进行试验，黑鲷每三天一次、金目鲈每两天一次，调查标识后鱼只的时间别残存率、时间别标识残留率以及水质资料，结果如下。

LDH release assay was improved and was compared with the crystal violet staining and the MTT colorimetry on sensitivity, repeatability and relateness.

对LDH 释放法进行改进，与结晶紫染色法和MTT比色法进行灵敏度、重复性和相关性比较。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Gram ' s stain A stain containing the basic dye crystal violet or gentian violet that forms the basis for the division into Gram positive and Gram negative bacteria.

革兰氏染色法：是一种使用结晶紫或龙胆紫作为染料来区分革兰氏阳性菌和革兰氏阴性菌的染色方法。

Research method: animal experiment method, dyeing HE method, histochemistry immunity method and statistics method .

研究方法：动物实验法，HE染色法，免疫组化法，统计学方法等。

Methods Tissue inorganic nitrate,malonaldehyde and NOS activity of the heart,liver,brain and kidney were determinated respectively by Cu2+-activated cadmium-reduction method,thiobarbituric acid test and oxyhemoglobin oxidation method.

采用铜离子活化镉还原法、硫代巴比妥酸染色法和血红蛋白氧化法测定组织NO、丙二醛含量和NOS的活性。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

To observe the effects of HASF on viability and collagen synthesis of two cell lines, AgNORs staining, 3H-proline incorporation and HYP determination were carried out.

层析法从兔羊水及胎兔血清中提取HASF并做活性鉴定；用嗜银蛋白染色法、3H-脯氨酸掺入法和羟脯氨酸测定法观察不同浓度的HASF对细胞增殖活性和胶原蛋白合成的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。