染色法

Pituitaries were stained by HE and immunocytochemical methods. Adrenocorticotropic hormone in rat blood plasma was measured quantitatively with radioimmunoassay.

对垂体进行HE和免疫细胞化学染色；应用放射免疫分析法检测血浆促肾上腺皮质激素含量。

Methods The embryo calvarial periosteum tissue was taken and, human osteoblasts were obtained by enzyme-assimilating methods. The morphological change, growth feature and osteogentic capability of osteoblasts were observel during culture in vitro, drew the growth curve was graphed and the cells was identified by alkaline phosphatase dye. At the same time, the morphology and bioactivity of 3 to 5 th-generation osbeoblasts and anabiotic cells were studied comparatively.

取人胚胎颅骨骨膜，采用酶消化法获取成骨细胞体外培养并传代，观察细胞形态，生物特性，绘制生长曲线，并经碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3~5代与冻存后成骨细胞的特点。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值，运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定，并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

It indicates that not only had FQ-PCR the characteristics of higher positive and accurative rates than smear acid-fast staining and antituberculotic antibody, but also had the advantage of accuracy quantitative analysis. It is of greater applicable importance that early diagnostic and medical treatment depend on FQ-PCR etiology data.

认为FQ-PCR技术不但具有明显高于涂片抗酸染色及ELISA—抗结核抗体法的阳性率和准确度，而且还具备准确定量分析的优点，为病人早期诊断与药物疗效观察提供了更为精确的病原学依据，具有更大的实用价值。

Methods The purified pancreatic PLA\-2 was injected intra arterially or intra cerebrally into SD rats and the actions of pancreatic PLA\-2 on the brain and spinal cord were ealuated.

方法对 SD大鼠进行 PL A2血管内及脑内注射，应用苏木精-伊红染色、髓鞘特染(Klurer and barrera，KB法)及电镜检查研究观察 PL A2 对动物脑脊髓的作用。

Methods INUS was used to measure the position, quality and the thickness of the plaque in the elastic artery and biopsy materials. After using HE and Hart stains, a micrometer was used to measuse the thickness of plaques and arterious walls.

以IVUS技术探测粥样斑块的位置、性质和大小，取材后进行HE、Hart法染色，用测微尺测量血管壁及斑块厚度，然后进行量化分析，辅以电镜技术观察病变的形态学改变。

Therefore, we believe that to 2% NaOH-NALC as a digestive juice, the concentration of magnetic nano-particles through fluorescent auramine O staining of a magnetic nanoparticle concentration and detection of TB than the traditional method of smear and culture have higher sensitivity.

因此，我们认为以2%NaOH-NALC作为消化液、用磁性纳米颗粒进行富集后通过金胺O荧光染色观察的一套磁性纳米颗粒富集TB并检测的方法会比传统涂片和培养法有更高的敏感度。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞，对原代至第5代rgcs进行细胞生长动力学分析，阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖，α sma和ⅱ型胶原的表达。

Materials and methods: Postoperative specimens from human parotid and submandibular glands were used. The glyoxylic catecholaminergic histofluores-cence method was employed on the tissues to study the sympathetic innerve-tion. One percent neutral red was used as a counterstain.

本研究以人类下颌下腺及腮腺切除的标本，应用甘草酸与儿茶胺形成之组织萤光法（glyoxylic catecholaminergic histofluorescence method），并采用1%中性红作对照染色，探讨2大唾液腺的交感神经分布。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。