染色法

Methods Epileptic animal models of adult Sprague-Dawley rats were induced by intraperitoneal injection of pilocarpine, which were divided into four groups including extrogenous NPY interference group.

方法将 SD大鼠随机分为 4组，采用匹鲁卡品癫痫动物模型及外源性 NPY侧脑室注入干预法，并以免疫组织化学染色观察 NPY的表达。

MethodsEarly embryos at different stages and uterus of the non-pregnant, and pregnant uterus of 1 to 8 days pseudopregnancy in mice were obtained.

方法取小鼠早期胚胎、动情期未孕、真孕及假孕1～8d小鼠子宫，利用免疫组织化学染色及图像分析法，检测p38MAPK在不同发育阶段早胚及围植入期子宫内膜中的表达和变化规律。

The relationship between the bile flow and cholecystokinin, gastrin,and secretin hormones in the rabbits after traumatic stress;2. Levels of GAS and EGF serum were determined by specific radioimmunoassay method.

观察维胃方对大鼠溃疡面积的改变；检测大鼠胃液pH值变化；通过石蜡包埋、病理切片染色观察溃疡愈合质量，并进行镜下分析；采用放射性免疫法检测大鼠血清表皮生长因子和胃泌素水平的变化。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

3 This section is taken from spinal cord of cat , silver-gilt

多极神经元(图7 3)本切片取自猫脊髓，镀银法染色。

Methods By immunohistochemical method, the expression of bcl-2 and bad gene were observed in 8 cases of normal breast tissues 19 cases of breast hyerplasia, 20 cases of breast atypical, 48 cases of breast cancer in situs ,and test the expression of ER , PR and bcl-2 , bad in the breast cancer, Eight normal breast tissues were used control.

利用免疫组织化学染色SABC法，原位观察19例乳腺单纯性增生，20例乳腺非典型增生，48例乳腺癌组织中bcl-2、bad蛋白的表达以8例正常乳腺组织作对照。

Highlights of the adjacent family room include a flat screen television, fireplace featuring Pennsylvania gray slate, and built-in bookcases with contrasting stain-grade backing.

聚焦相邻家庭房间包括平板电视，壁炉采用宾夕法尼亚灰色板岩，并内置书柜与对比染色级支持。

There were inflammatory cells infiltrating in the myocardium sparely and no calcification in the pericardium in IVIG and IVIG+VitC groups. The size of spleens enlarged especially in IVIG and IVIG+VitC groups.

心脏分为三部分：一部分作病理切片和H-E染色，一部分作冰冻切片，在荧光显微镜下用直接免疫荧光法测定心肌组织中沉积的IgG水平，一部分作电镜检查。

Establishment of a bovine mammary tissue culture system Bovine mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation.

1奶牛乳腺组织体外培养方法的建立采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织，通过LDH活力测定、台盼兰染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响，建立奶牛乳腺组织的培养体系。

Gene bcl-2 and bax expression in tissue sections were assayed with immunohistochemistry supersensitive kit with a streptavidin-biotinperoxidase complex method.

应用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物法免疫组化染色超敏试剂盒检测血管壁bcl-2、bax基因表达情况。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。