染色法

METHODS: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups was observed by histological examination. The expression of proliferation cell nuclear antigenl and α-smooth muscle actin were determined by immunohistochemistry. The content of nitric oxide was measured by nitrate reductase chromatometry.

建立大鼠腹主动脉移植慢性排斥反应模型，分为3组：异系移植对照组、异系移植治疗组和同系移植组，60d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度；并行免疫组织化学染色，检测移植动脉增殖细胞核抗原和平滑肌肌动蛋白的表达情况，行硝酸还原酶比色法测定移植动脉组织NO的含量。

In order to study on the QSRR of fatty esters, 100 fatty esters were optimized by semiempirical calculations AM1, then calculated them by quantum chemistry algorithnl and the technique of molecule graphics and AM1 to acquire the Kier index and Kappa shape index.

为了研究脂肪酯的构效关系，应用半经验量子化学AM1法得到了100种脂肪酯分子的优势构象，利用量子化学算法和分子图形学技术获得Kier指数和Kappa形状指数，再在分子拓扑理论的基础上，提出了一个修正的拓扑染色指数mL，用多元回归技术建立了脂肪酯化合物的结构与色谱保留值的相关性模型。

In light and electronic microscopy, they appeared cell shrinkage, cell density increasing and eosinophilic, aggregation of cytoplasmic organelles, nucleolus fragmentation, and chromatin margination, condensation, and fragmentation. Those changes could be detected by TUNEL method.

在光镜和电镜下癌细胞凋亡表现为细胞体积缩小，胞浆浓缩嗜酸性，胞浆出泡、细胞器缩小或减少，核仁松解，染色质聚集、靠边、浓聚、断裂，在TUNEL法检测凋亡癌细胞时也可观察到相应的形态特点。

MAIN OUTCOME MEASURES: Methods of collagenase digestion,microdissection and results of histochemical staining.

主要观察指标：胶原酶消化法、立体解剖分离法及组织化学染色结果。

Then, the brains were taken out after anesthesia and cut on a microtome. The number of PV-positive neurons in the basal forebrain was analyzed by using ABC immunohistochemical staining method and image analysis.

麻醉后取脑制备组织切片，ABC法免疫组化染色结合图像分析观察各组大鼠基底前脑小白蛋白阳性神经元的变化。

Aortic perfusion of a fixative solution. Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidin????

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化；②海马的nNOS免疫组化研究：PSD 24，48和72 h后，立即取脑、固定、行冰冻切片（片厚25 μm）、SABC法免疫组化染色、光镜下进行观察分析、摄像。

Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidinbiotinperoxidase complex technique.

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化；②海马的nNOS免疫组化探究摘要：PSD 24，48和72 h后，立即取脑、固定、行冰冻切片（片厚25 μm）、SABC法免疫组化染色、光镜下进行观察分析、摄像。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

We select 5 cirrhotic tissues and 5 HCC with their paired samples for tissue transplantation and cultured fibroblasts.

免疫组织化学染色ABC法检测三种组织样本中c-kit、CD133、CD90、CD34、CK18和CK-19等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。