染色法

Histopathological changes of lung tissue were observed under light microscope. The protein expression of MMP (MMP-2, MMP-9) and TIMP (TIMP-1) in lung tissue were determined by immunocytochemistry. RESULTS: Decompensate respiratory acidosis (pH =7.20±0.04, PaCO2 =7.84±0.15) developed in group B.

用ELISA法测定大鼠血清MMP-2、MMP-9、TIMP-1活性及NE活性；弹力纤维染色标本进行弹性纤维相对含量的测定；免疫组化方法检测肺组织中MMP-2、MMP-9及TIMP-1的表达及光镜下进行病理学观察。

The expressions of bFGF in CA1, CA3 and dentate gyrus areas of hippocampus were detected by immunohistochemistry method.

免疫组化法观察各组海马CA1、CA3区及齿状回bFGF表达，HE染色观察病理形态学改变。

We identified the chromosomal number of Dracaena cambodiana for the first time and developed the techniques for observing chromosomes in the root-tip somatic cells.

改良石炭酸品红法要点是于上午9时~10时采集根尖，用饱和的对二氯苯预处理6 h，固定4～18 h，置于1 mol/L稀盐酸在60℃恒温水浴解离6 min，置载玻片用改良石炭酸品红染色30 min，敲片后显微观察。

Methods Immunohistochemistry and pathologic image analysis were used to semiquantitatively detect the expression of EGFRvⅢ protein in eighty human suprarenal epithelioma tissues and twenty-four normal renal tissues.

采用免疫组织化学染色和图像分析法检测80例肾透明细胞癌和24例正常肾组织中EGFRvⅢ的表达水平，进行统计学分析、比较。

SLNs of 48 patients with early-staging breast cancer were detected by methylene blue staining SLNB with intraoperative frozen section combined with rapid immunohistochemical staining by EPOS. 28 patients were found negative, and were randomized to receive simplified ALND(n=15, experiment group) or standard ALND(n=13, control group).

30例早期乳腺癌行美蓝染色SLNB术中行SLN冰冻切片联合EPOS法快速免疫组化检查，其中阴性者28例分成两组，15例行简化的ALND(仅清扫胸小肌外侧组淋巴结，简化组)，13例行标准ALND。

He expression of the multidrug resistance gene product P170 and the PCNA (proliferating cell nuclear antigen) was analyzed in 67 bone malignant tumours, 15 normal bone tissues and 15 exostosis by S-P method immunohistochemistry.

作者采用S－P法免疫组织化学染色技术观察67例恶性骨肿瘤组织、15例正常骨组织、15例骨软骨瘤组织中多药耐药基因产物P糖蛋白（简称P170）、增殖细胞核抗原的表达情况。

Methods The double flourescent staining of apoptosis and Desmin was examined in heart tissue of Keshan disease by immunohistochemical and TUNEL,and the confocal microscope was used to observe the results.

应用原位末端标记法及免疫组化技术在亚急型和慢型克山病患者心肌石蜡包埋组织切片上进行细胞凋亡及Desmin双重荧光染色，并应用共聚焦显微镜观察结果。

Analysis of high-temperature dyeing in the performance management process, through large amounts of data and statistics, the use of the Hungarian method and fuzzy comprehensive evaluation, and other management theory, the objective of the department staff and the performance of the quantitative analysis and made a The views of some improvements, the business performance management and development of in-depth work, a very important role.

在分析高温染色绩效管理难题的过程中，通过大量的数据统计与计算，运用了匈牙利解法和模糊综合评判法等管理学理论，客观的对部门及员工的绩效进行了量化分析，并提出了一些改进的意见，对企业绩效管理工作的深入和发展，有着极其重要的作用。哪位有英文文献，英汉都有帮助粘贴点！！！！！！！！

There are collagenous fiber in body integument especially the ventral integument and the hymenopterous integument discovered by the H.E.

用伊红和马洛赖氏三色法染色可见体壁尤其在腹壁的折叠处及膜翅壁中存在着胶原纤维。

Methods BALB/c mice were anesthetized with ether and infected intracranially with 20 μl 4.5×108 plaque forming units of coxsackievirus B3. CVB3 RNA in mice brain was detected by reversal transcription polymerase chain reaction and the pathological changes of brain tissues were observed by HE staining.

BALB/c纯系小鼠分两组，实验组小鼠颅内直接注射滴度为4.5×108 pfu的柯萨奇病毒B3悬液20 μl，对照组颅内注射生理盐水20 μl，应用逆转录酶聚合酶链反应法测定脑组织中CVB3 RNA含量，HE染色观察脑组织病理学的改变。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。