染色法

Methods: Seventy-eight specimens of disc herniation(classified as protrusions,, extrusions, and sequestrations) and fourteen nonherniated discs were immunostained with antihuman MMP-3 monoclonal antibody, using the avidin-biotin-peroxidase complex method.

运用免疫组织化学抗生物素蛋白—生物素—过氧化物酶复合体法，通过抗MMP3的单克隆抗体，对78例椎间盘突出症和14例非椎间盘突出症的椎间盘标本进行染色。

Antibodies including mouse antihuman neurofilament,rabbit anti-human myelin basic protein,rabbit anti-human Al,rabbit anti-human IgM,mouse anti-human IgG,mouse anti-human CD31,mouseanti-human GFAP,mouse anti-human Ki-67,rabbit anti-human serotonin(5-HT),rabbit anti-human SYN,and mouse anti-human c-fos protein were used for immu-nohistochemistry with S-P method.The colorant was brown yellow DAB.

免疫组化染色用S-P法，一抗分别为鼠抗人神经丝蛋白、兔抗人髓鞘碱性蛋白、兔抗人Al、兔抗人IgM、鼠抗人IgG、鼠抗人CD31、鼠抗人GFAP、鼠抗人Ki-67、兔抗人血清素（5-HT）、兔抗人SYN及鼠抗人C-fos蛋白，显色剂为棕黄色DAB。

The emulsion polymerization was used to prepare the fluorine-contained resin with butyl acrylate and methacrylic hexafluoro butyl ester as monomer,so as to improve the apparent shade depth of fine-denier dyed polyester fabric.

以丙烯酸丁酯和甲基丙烯酸六氟丁酯为单体，采用乳液聚合法合成含氟树脂，用于提高涤纶染色织物的表观深度。

The neural complex of ascidian Styela clava was observed by two histological methods, and the methionine-enkephalin in the neural complex of Styela clava was analyzed by the immunohistochemical S-ABC (strept avidin-biotin complex) technique,respectively.

采用甲苯胺蓝染色和Golgi镀银法对柄海鞘神经复合体的结构进行显微观察，并通过免疫组织化学方法对甲硫氨酸脑啡肽在神经复合体中的分布进行了研究。

Abstract］ Objective To explore the effect of radiotherapy on the auxesis of Hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.Methods After radiotherapy with different doses,the Hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.

目的 探讨放射对宫颈癌Hela细胞系再增殖的影响，宫颈癌是否有必要实行非常规分割放疗提供定的理论基础方法在不同剂量的放射作用下，以台盼蓝染色细胞计法分析Hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Abstract］ objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results （1）tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响，为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下，以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化；以克隆形成实验检测其同期克隆源性细胞存活比率，并将两者结合分析。

Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2％TTC染色，用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比；取视交叉前平面梗死边缘区皮层组织约100mg，用酶消化法制作神经细胞悬液，用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡，以细胞平均的荧光强度代表细胞内钙浓度；冰上迅速分离视交叉后梗死边缘区皮层组织约100mg，于低温下制作脑组织匀浆，用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

The SAH animal model was established by autologus arterial blood injection into the cisterna magna twice.

采用枕大池2次注血法制作SAH的动物模型，并进行CGRP和ETRA的免疫组化染色，观察CGRP和ETRA的免疫表达。

Immunohistochemistry and imaging analysis were used for detecting the intensity of expression of u-PA and PAI-1 in renal biopsied tissue of 50 cases with various types of LN and 10 cases with minor glomerular lesions, the results were analyzed correlatively with PCNA cells and type IV collagen.

方法对50例不同类型的LN和10例原发性肾小球轻微病变的肾活检组织，采用免疫组化及图象分析法比较观察了u-PA和PAI-1在肾小球的表达强度，并与肾小球增殖细胞核抗原阳性细胞数和Ⅳ型胶原染色强度作相关分析。

Based on the characteristics of molecular structure, the adjacent matrix and dyeing matrix were used to characterize the molecular structure of alkane and cycloalkane, a new method——the group contribu-tion method, was developed to calculate the boiling points of alkane and cycloalkanes from molecular struc-ture.

根据分子结构的特点，通过用邻接矩阵和染色矩阵表征分子结构，发展一种根据分子结构信息计算烷烃和环烷烃沸点的新方法——基团贡献法。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。