染色法

Equal amount of absolute alcohol was given to the control group. The expressions of vitamin D receptor in HL-60 cells of each group were identified by Western blot analysis, and cellular morphological changes were observed after being stained by Wright-Giemsa.

Western印迹技术鉴定维生素D受体在各组HL-60细胞的表达；Wright-Giemsa染色观察细胞形态学变化；流式细胞术检测细胞表面标志物CDl4抗原的表达；反转录-聚合酶链反应法分析c-myc基因的表达。

The percentage of through the platform position was calculated. The learning and memory behavior was observed mainly in rats of every group. After the experiment, sodium pentobarbital was injected by intraperitoneal injection of all the rats for anesthesia, and the blood was gained from heart.The plasmcortisol and adrenocorticotrophic hormone were detected by the radioimmunoassay.

取模型组大鼠8只，对照组大鼠5只，不经灌注固定，冰台上迅速分离大脑，将大脑沿矢状缝分左右半球，左半球用于糖皮质激素受体mRNA原位杂交，右半球采用薄层扫描法测定谷氨酸和γ-氨基丁酸含量，同时取大鼠左侧肾上腺进行苏木精-伊红染色，观察肾上腺组织形态变化。

Sixteenth week after operation, the animals were sacrificed by aeroembolism. Cartilage grading was observed by general observation and operation microscope. Specimens were procured from the weight-bearing portion of the femoral condylar, and evaluated according to a modified histological-histochemical grading system (0-1 point as normal and over 10 points as severe) by haematoxylin-eosin and Safranin'O/Fast Green stainings.

术后第，6周空气栓塞法处死动物，大体观察及手术显微镜下观察软骨表面分级；取股骨髁负重面软骨标本进行苏木精－伊红、沙黄染色及骨关节炎组织病理学评分（正常为0~1，重度为10分以上）、分级。

There was a linear positive correlation between the alkaline phosphatase activities and cell density.

液氮冷冻保存后复苏培养复层成骨细胞钙钴法碱性磷酸酶染色阳性率在45%以上，超微结构显示为高分化功能活跃成骨细胞。

The cells were round before adherence,and anomalism fusiform,triangle and polygon when adhered,monocaryon,1-3 nucleole by invert phase contrast microscopy observation.Alkaline phosphatase staining and mineralized nodules staining were positive(≥98.06%).The osteoblastic activity was the highest on day 10,which was observed by MTT and morphological method.

倒置相差显微镜形态观察结果显示，未贴壁的细胞呈圆形，贴壁生长的细胞呈不规则梭形、三角形或多角形，单核，有1~3个核仁；碱性磷酸酶和钙化结节染色均呈阳性（阳性率≥98.06%）；MTT法结合形态学观察结果显示，成骨细胞培养至第10d时活性最强。

METHODS: HUVECs were identified by microscopic observation of morphology and immunoflourescence staining with a monoclonal mouse antihuman vWF antibody.

采用胰蛋白酶消化法体外分离健康人脐静脉内皮细胞，通过光镜下形态观察和抗vWF免疫荧光染色对培养细胞进行鉴定。

Possible sources of steam and condensate infusion may be found through visual examination,fluorescent dye penetrants,or air forced into the outside surface while adiacent internal surface are coated with a soap film.

蒸汽和冷凝液泄漏源的查找方法是：目测法和荧光染色渗透剂法，或者是（通过具体现象进行判断：）空气进入外层缸体，而附近的内表层却被一层肥皂膜所覆盖。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠，血清进行肝功能检测；苏木素－伊红染色部分肝组织；Trizol-酚－氯仿一步法提取小鼠肝组织总RNA，紫外分光光度法测定总RNA浓度及纯度，实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达，以18S基因作为质控。

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量；罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位；超氧化物歧化酶检测试剂盒检测SOD活性；免疫印迹法检测血红素氧合酶－1(HO-1)的表达。

The acid phosphetase in the blood cells of oncomelania hupensis was observed at the light microscope level by the burstone stained technique.

将解剖的钉螺软体组织经挤压法获取钉螺血淋巴液，以偶氮色素法染色后，光学显微镜下观察螺血淋巴细胞酸性磷酸酶活性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。