染色法

In the latter, only reds, blues and browns registered and there was only static camerawork and no feel for muting or shading of colors.

关于染色的方法我也不太懂，但我知道这种"彩色染印法"肯定比更早出现的胶片"手工着色法"复杂得多。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度，并取后极部巩膜组织，用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞，应用免疫组织化学染色和蛋白印迹（Western-blot）法检测bFGF和bcl-2蛋白。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

The root systems were collected with mesh bag method and nematodes in root were stained by the method of fuchsin acid–NaCI...

采用网袋法收集根系，酸性品红-次氯酸钠法对根染色，体视显微镜下统计根内各龄期线虫数量。

Materials and Methods: Forty Sprague-Dawley rats were randomly divided into normal group, control operative group and operative group which includes 6 subgroups (1,3,7,14,21,28 days). The spinal cord hemisection injury model was made by cutting the posterior right side spinal cord. Function changes in the right posterior limb were evaluated by BBB method. The general morphology changes were observed by HE stain.

材料与方法：40只成年SD大鼠随机分为正常组，假手术组和手术组，手术组又分成1，3，7，14，21，28天组，选取制作脊髓右侧半横断损伤模型，BBB法进行右下肢功能评分，HE染色观察损伤脊髓不同时段的大体形态学变化，原位杂交法观察slit-2 mRNA的表达变化，计算阳性细胞灰度值，进行统计学分析。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究；通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究；运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况，对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

The observation of macrography, histology (stained by HE, safarnin O and toluidine blue), transmission electron microscope, scanning electron microscope and the method of TUNEL were made for the three groups which were killed postope- ratively at 6 hour,4 week and 24 week.

将常规HE和番红"O"染色软骨病理切片采用双盲法阅片，按照Mankin评分法对软骨冲击伤的严重度给予组织评分。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。