染色法

Results The amaranthine nuclei had a distinct outline and distributed evenly on the slide. The area of nuclei in liquidbased preparation was much smaller and chromatin was more condensed in comparison with those in conventional smear. The nuclei in conventional smears had the largest value of area and the value of AOD, and CV of IOD, the IOD ratio among diploid, tetraploid and octaploid DNA was most close to 2 and 4, and the CV of IOD exceeded 6%.

结果 三种涂片内肝细胞核分布均匀，轮廓清晰，呈紫红色，与传统涂片法相比，液基法内肝细胞核面积明显减小，染色质高度浓缩；相同倍体的肝细胞核在传统涂片中面积最大，平均光密度最低，各倍体间的比值最接近2和4，积分光密度CV值最低（。5）。

The osteogenic differentiation markers were determined by para position Nitrobenzene phosphoric acid and Alizarin Red S staining.The expressions of CBFA1 and VEGF mRNA in BMSCs were detected by RT-PCR.

采用改良MTT法测定含药血清对P2代BMSCs增殖的影响；用对硝基苯磷酸法和茜素红S染色定量法对BMSCs分化为成骨细胞的标志物进行测定。

Methods:1100 of positive sputum specimens smears were heated by magnetic hotplate.The result of microscopic examination was compared with the traditional acid-fast stain smear.

应用电磁炉加热法对1100例阳性痰标本涂片抗酸染色镜检，所得结果与传统的抗酸染色镜检结果进行比较。

FCM appeared obvious inferior diploid peak and blocked the cells to the G_1 phase.The red particles throughout the cytoplasm and permeated the plasma membrane could also be seen.Chromatin lining and breeding body could be seen under the transmission electron microscope.

FCM出现明显的亚二倍体峰并将细胞阻滞于G1期，Annexin-V-FITC联合法染色可见细胞凋亡的特征性贯穿胞质穿过胞膜的红色颗粒，电镜下可见染色质边集及生发小体的凋亡形态特征。

Methods BTXA labeled with125Iwas obtained. The radioactivitymeasurementwas taken tomeasure the solubility ofBTXA in gel and BTXA solution. The rightgastrocnemiusmuscles of12 SD ratswere injectedwithBTXA geland the leftgastrocnemiusmuscles injectedwith normal salinewere used as controls. HE staining and 1% gold chloride stainingwere used to study the tissue changes 6 months and 12 months afterBTXA gel injection and transmission electronmicroscopy was used to observe the ultrastructural changes.

将BTXA行示踪标记配制125I-BTXA水凝胶与125I-BTXA水溶液，进行样品放射性强度测定。12只SD大鼠用随机数字表法分为A、B两组，每组6只，大鼠右侧腓肠肌注射BTXA水凝胶，左侧腓肠肌注射等体积生理盐水作对照，于注射后6个月、12个月切取腓肠肌标本，常规病理HE染色、1%氯化金染色，透射电子显微镜观察组织结构变化。

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

The rabbit osteoarthritis model were duplicated by Hulth's technique. The pathologyical changes of each group was observed with naked eyes before taking the thighbone department carilage of pulley block and the platform place of shine bone carilage, then under the light microcope and the transmission electron microcope respectively, after treating with Semer Strychni (high、middle and low dose) for two months.

按Hulth法造兔膝骨关节炎模型，用马钱子高、中、低剂量进行灌胃治疗，连续2月后取材，进行肉眼大体观察后，取股骨滑车部软骨及胫骨平台处软骨，分别于HE染色后光镜下观察和铀-铅双重染色透射电镜下观察各组的病理变化。

An initial probe into the single dye vat is given.The results have shown that:the double dye vat can raise color fastness in wool dyeing and can strengthen gorgeous.The color fixing agents AC estimate is 6%.

结果表明，采用二浴法染色固色可明显提高毛线产品的色牢度，而且可使产品增亮增艳；固色剂AC用量以6％为宜；染色后应充分漂洗以除去浮色，待毛线烘干后放置一段时间，其牢度更佳。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

36 big mice stochastically divide into the vacation surgery group, castrate the group and castrate the sterandryl substitution group.

线栓法建立大鼠局灶性脑缺血再灌注损伤模型，TTC染色测脑梗死体积及免疫组化染色检测脑组织Bax的表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。