染色法

Results Trunk neural crest stem cells were successfully isolated and cultured. The immuocytochemcal result showed that the cells were nestin-and P75-positive. Trunk neural crest stem cells grew through adhering to chitosan fiber after inoculation. Under an electon microscope, the spindle-shaped trunk neural crest stem cells were proliferated and migrated along the fiber. The cells arranged side by side or linked by heads and tails in processes. The ends of S-100 positive cells were flat and expanded, adhering to the fiber as claw-shaped pseudopodium.

结果 采用神经管植块法培养的神经嵴干细胞，免疫细胞化学染色呈现nestin及P75双阳性，将其接种在壳聚糖纤维支架材料上，可见神经嵴干细胞贴附于壳聚糖纤维上生长；S-100免疫细胞化学染色显示，S-100阳性细胞突起的末端呈扁平状膨大；扫描电镜显示，壳聚糖纤维上的躯干神经嵴干细胞为梭形，呈现"端对端"、"肩并肩"排列，伸出爪形伪足贴附于壳聚糖纤维上。

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

muscles biopsy of 25 patients with lipid storage myopathy were freezed and cut into slices. Specimens by routine histology and acid or alkaly myosin ATPase, oil red O and PAS stain were observed the difference of types of keletal muscle fibers by ATPase stain and degree of fatty and glycogen dispersed in sarcoplasm,explored substancs metabolite .

方法将25例LSM患者活检骨骼肌作为研究材料，做冰冻切片，采用常规组织学和酸、碱预孵育肌球蛋白ATPase法、油红O法、PAS法观察各型肌纤维ATPase染色差别及肌浆内糖原和脂肪的分布，以探讨其物质代谢。

AgNORs protein stained by silver were used in cytology with Kappa of inter-observers 0.81. For the USG, the patients were scanned with trans-rectal probe with Kappa of inter-observers 0.76. The results of urine cytology combined with USG （Positive when urine cytology and/or USG positive.

采用盲法对每一纳入研究对象同时进行尿脱落细胞嗜银染色、经直肠B超和膀胱镜检查，以膀胱镜结合病理检查的结果为金标准，计算尿脱落细胞嗜银染色结合B超联合诊断膀胱癌经尿道手术后复发的敏感性、特异性和ROC曲线下面积。

RESULTS: The levels of toxicity ranged from 0 to 1 grade. HE staining and toluidine blue staining demonstrated that PDLCs could adhere and proliferate on the surface of SIS in good condition.

结果：MTT法检测证实细胞毒性为0~1级，材料无细胞毒性；HE染色和甲苯胺蓝染色可见PDLCs在SIS表面和内部均生长良好，显示SIS具备良好的生物相容性。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

Starting from the solubility of disperse dyes, the solubility, up-taking rate, migrating rate of dis_perse Yellow M-4G, Red 3B and Blue 2BLN at different concentration of Leveling Agent GS were tested by poly_mer thin-film bag method, thus demonstrating the hyperchromic effect and achromic effect of leveling agent during dyeing with disperse dyes.

从分散染料溶解度的角度出发，采用聚合物薄膜袋法测定了分散黄M－ 4G、分散红3B、分散蓝2BLN 三种分散染料在匀染剂GS 不同浓度时的溶解度以及上染率、移染率，从而展示了匀染剂在分散染料染色中的&增色效应&和&消色效应&，并从染色理论上推论了产生这两种效应的原理。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Then the graft was taken out for further use. MAIN OUTCOME MEASURES: Hematoxylin-eosin staining and Azon staining were used for morphological observation.

主要观察指标：以苏木精-伊红染色和Azon法染色后进行形态学观察。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。