染色法

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Methods Beecher tissue arrayer was used to create a196-dot tissue microarray,derived from99cases of common carcinomas,and their para-cancerous tissues and corresponding normal tissues which were formalin-fixedand paraffin embedded.

在病理档案材料中选取10种常见恶性肿瘤及其癌旁或正常组织的石蜡包埋标本，HE染色切片定位穿刺部位，每一蜡块穿取直径0.6mm大小组织柱，排布成196点列阵的芯片；用Ki-67，p53，rasp21，nm-23和E-cadherin单抗和S-P法进行免疫组化染色，对各切片阳性细胞进行计数观察。

ResultsA slide of bacterial suspension with a concentration of 1.5×1012/L , to be placed at 37 ℃ for 10 min then stained by carbolfuchsin for 10-15 min. Under a microscope, a clear background with red thalli and faint red flagellum could be seen in 80%-90% of the bacteria.

结果 浓度为1.5×1012/L的菌悬液37 ℃放置10 min制片，用石炭酸复红法染色10～15 min，水洗，干燥镜检，其背景清晰，80%～90%的细菌有鞭毛，菌体红色，鞭毛淡红色，染色效果理想。

The results indicated that chromosome counting was the most reliable and precise method, however, it was cumbersome and required skilled cytological techniques; The chromocenter size, heterochromatin number, chroloplast number of guard cell and certain plant morphological characteristics are simple and practical methods for ploidydetermination but with some shortages.

结果表明：对体细胞染色体的直接计数法是最可靠的方法，但费时、难度大；叶片气孔大小与倍性无显著相关性；保卫细胞叶绿体数目、体细胞染色中心大小以及异染色质个数与黄瓜倍性密切相关，是简单、快速而有效的倍性鉴定方法；植株的形态学观察也能间接确定植株倍性，但鉴定时期偏晚，精确性较差。

Cell of Ela of 鳫 of fleeing Yu Piao, the stage longs for blue refus to catch a law to detect wither of cell of revulsive Hela of EVn-50 of observation of microscope of fluorescence of coloring of cellular vigor;AO/EB dies electrophoresis of gel of morphology change;DNA is corroborant wither of cell of EVn-50 revulsive Hela dies action;PI coloring sheds type cell appearance to detect wither of cell of EVn-50 revulsive Hela dies rate.

体外培养Hela细胞，台盼蓝拒染法检测细胞活力；AO/EB染色荧光显微镜观察EVn-50诱导Hela细胞凋亡形态学改变；DNA凝胶电泳确证EVn-50诱导Hela细胞凋亡功能；PI染色流式细胞仪检测EVn-50诱导Hela细胞凋亡率。

METHODS: Total 60 male Wistar rats were randomly divided into 6 groups, i.e., normal control group , DMN model group , large dose treatment group , middle dose treatment group, small dose treatment group and Dongbao gantai treatment group. The liver fibrotic model was induced by i.p. dimethylnitrosamine for 4wk. The relative herbs were given after model establishment in each treatment group and the administration lasted for 6 weeks. Decollate to get the blood and The serum hepatofibrosis indexes, hyaluronic acid, collagen IV, pro-collagen type III and laminin were measuredwith radioimmunoassay. SOD and MDA were measured with biochemistry method. The liver pathological changes were observed in each group of rats.

将60只雄性Wistar大鼠随即机分正常对照组、模型对照组、实验大剂量组(20ml/kg/d)、实验中剂量组(10ml/kg/d)、实验小剂量组(5ml/kg/d)及东宝肝泰药物对照组；腹腔注射二甲基亚硝胺制备大鼠肝纤维化模型，连续灌胃6周，断头取血，生化检测血清SOD、MDA；放射免疫法测定血清HA、LN、PCIII及IV-C；取肝左叶做HE染色和Mallory胶原染色，观察肝病理组织学改变。

Comparing the results of various sequences of double label should that the sequence of choice is PAP→ABC-HRP in double immunoperoxidase stain and PAP→ABC-AP and ABC-HRP→ ABC-AP in double immunoenzymatic stain.

通过比较酸洗脱法、氧化法、PBS直接漂洗法对胰岛细胞双重免疫组化染色的影响，发现三种对一染抗体的处理方法无显著的差异。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

Results (1)The permillage of myocyte apoptosis in infarct area in elder patients with sudden death caused by AMI was significantly higher than that in control group(663.00‰±117.12‰ vs 34.30‰±20.68‰,P＜0 .01).However the permillage of myocyte apoptosis in 1-vessel,2-vessel,and 3 - vessel disease were 514.28±165.12‰,564.38‰±102.33‰ and 668.25‰±127. 19‰,respectiverly,significantly higher as compared to control group(All P＜ 0.01).But no significance was found among the three groups.(2)The size of DNA f ragment about 180～200 bp was found only in those patients with two and three ve ssels involoved.(3)The electron microscope showed the characteristics of myocyte apoptosis episodes,the others showed the characteristics of necrosis.

结果 TUNEL法发现，猝死者梗死区的心肌细胞凋亡千分数老年组(663.00‰±117.12‰)明显高于正常对照(34.30‰±20.68‰)(P＜0.001)；心肌细胞凋亡千分数在冠脉1支病变者(514.28‰±165.12‰)＜2支病变者(564.38‰±102.12‰)＜3支病变者(668.25‰±127.19‰)，虽然均明显高于正常对照(均P＜0.01)，但三组之间比较则无统计学上的差别(均P＞0.05)；冠脉2～3支病变者梗死区的心肌细胞DNA电泳可见相差约180～220 bp的阶梯片段；电镜发现猝死者梗死区内的心肌细胞核膜完整、染色质浓集、电子密度增加的凋亡特征，有的则出现核膜破裂、染色质溶解成碎屑的坏死现象。

Objective: To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification. Methods: Using 7 different types of primary antibodies and 2 tomor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining sod compared their sensitivities and specificities. Results: The modified CSA method was the moat sensitive one among the 4 methods.

目的：探讨酪胺与过氧化物酶发生酶促反应后，用热缓冲液洗以进一步提高和改善催化信号放大法的敏感性和特异性方法：应用7种不同类型的一抗，2张不同肿瘤的组织芯片，同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色，比较敏感性和特异胜。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。