染色法

The results showed:(1) the cellule morphous and the disposition in chick embryo of PGCs were coincident no matter stained by PAS or HE staining, and HE staining could disclose the morphologic characteristics more clearly, exactly and completely;(2) genesis of blood island could be observed at the boundary of light and dark region of the extraembryonic blastoderm at about 26 hours;(3) both the blood vessel endothelium cells and free cells of the blood island were differentiated from PGCs. The generating of genuine yolk sac was at about 44-48 hours.

结果表明：①用经典的识别原生殖细胞的PAS染色方法与HE染色所观察到的PGC在鸡胚的分布及细胞形态基本一致，而HE染色较PAS法能更有助于清晰、完整、准确地识别PGC的细胞内部结构特征；②在孵化26小时的鸡胚胚外胚盘的明暗交界处可见血岛的发生，血岛发生的时间比真正的卵黄囊产生时间（大约44-48小时）要早很多；③血岛周围的血管内皮细胞与管腔中游离细胞都来自PGC。

Or cytologic examination, the samples were prepared using standard techniques. Sample for FCM analysis were centrifuged and exposed to hypotonic solution cotaining detergent and propidium iodide. Thirty-eight patients had pleural effusion due to benign disease, whilst 33 patients had primary lung cancer. All 38 patients with benign pleural effusions showed FCM diploidy.

CM之DNA定量法是测定细胞是否为不正常DNA含量，其染色是用propidium iodide染色，临床细胞诊断法是用Dapanicolaou染色方法，其中38位病例之胸水原因是良性病变，33位胸水病例则有原发性肺癌，38位良性胸水病例之FCM DNA定量结果全部为正常含量。

E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

INTERVENTIONS: 4 vessel occlusion(4VO) brain ischemic models in rats stained with thionine staining and GFAP immunohistochemistry staining. were used. Sixty-four rats, of which bilateral vertebral arteries were occluded permanently by electrocautery, were divided into the following 8groups: sham operation group, cerebral ischemic preconditioninggroup, ischemic insult group; BIT group; MTPG + sham operation group;MTPG+BIT group; MTPG+ischemia group and -4C3HPG+BIT coup. All the rats were killed 7 days after the operation or the final ischemic treatment. Cerebral sections were selected and stained with thionine staining and GFAP immunohistochemistry staining.

干预：采用大鼠四血管闭塞全脑缺血模型，应用硫堇染色和胶质纤维酸性蛋白免疫组化法。64只大鼠椎动脉凝闭后分为假手术组、单纯预处理组、单纯缺血组、脑缺血耐受组，MTPG+假手术组、MTPG+脑缺血耐受组、MTPG+缺血组和-4C3HPG+脑缺血耐受组，所有动物均在手术后或末次缺血后7 d处死取材，行脑组织切片，硫堇染色和胶质纤维酸性蛋白免疫组化染色。

Then the content of the interleukin (IL-1) in serum were measured by the method of enzyme-linked assay; From the HE dyeing, the change of morph in the arthrodial was observed by the light microscope; From the AB-PAS dyeing, the change of PG in the cartilage matrix was observed; By all of that the therapeutic efficacy of the medicine wine Yong GuKangNing to the disease of osteoarthritis was estimated, and the mechanisms to the disease was also investigated.

用酶免法测定血清中白细胞介素(IL-1)的含量；常规HE染色，光镜下观察关节软骨结构形态学变化；AB-PAS染色，光镜下观察软骨基质中蛋白聚糖的染色变化；评价永康宁骨康宁对膝骨性关节炎的治疗效果，并探讨其作用机制。

The optimal process parameters and conditions were introduced as following: the best stripping effect with dosage of blankit 2.0g/L and one bath redyeing process with disperse and ANOSETWPN acid dyestuffs at 105~115℃, 15~20min.

实验得出，漂毛粉浓度控制在2.0g/L时，剥色效果最佳；染色选用ANOSEWPN酸性染料，采用分散／酸性－浴法进行染色，染色温度105~115℃，保温15~20min。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究：对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色；并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色，CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

Single factor method were compared to the concentration of dyes, dye bath pH, temperature, Dyeing time to do a series of gradient experiment, the two groups reached on experiments with suitable for dyeing process conditions,such as the best dye pH, suitable dye concentration, Dyeing appropriate time,temperature values of humic acid and fulvic acid were dyeing wool and silk, and which the general situation has deep understanding.

采用单因素对比法分别对染料的浓度、染液pH值、染色温度、染色时间等做了系列梯度实验，得出了两组上染实验的最佳染液pH值、染料合适浓度、适当染色时间、适宜上染温度等工艺条件值，对腐植酸和黄腐酸分别上染羊毛线及真丝绸的大体情况有了很深的了解。

After treated in 4% paraformaldehyde,we applied Hematoxylin-Eosin and Nissl staining to exhibit the structure of the retinae and ganglion cells respectively,and also we used immunohistochemical ABC method to display the distribution of GFAP-immunoreactive cells that were specifically classified as the astrocytes.

E 染色以显示视网膜结构，Nissl染色显示神经节细胞，免疫组织化学ABC法染色以显示星形胶质细胞特征性标志物胶质纤维酸性蛋白的阳性反应细胞的分布。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。