染色法

MAIN OUTCOME MEASURES: Proliferation and osteogenic activity of cells were measured by morphology, ultrastructure, and cell proliferation curves, analyzed by calcification nodi Von Kossa staining, and determend by quantitative analysis of contents of cytoplasmic alkaline phosphatase and amendable calcium-cobalt staining.

主要观察指标：通过形态学、超微结构，细胞增殖曲线，钙结节Von kossa法染色以及细胞内碱性磷酸酶定量检测与钙钴法染色确定其增殖与成骨活性。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

Apoptosis of lymphocytes in bone marrow, spleen, thymus and bursa was detected by light microscope, electron microscope and terminal- deoxynucleotidyl transferase UIP nick end labeling. The results showed ALV-J could induce incoordinate apoptosis of lymphocytes in thymus, bursa and spleen, which was mainly found in early time. And the apoptosis was much more obvious in thymus than in other immune organs.

以电镜观察和HE染色、原位末端标记染色光镜观察对ALV-J感染和混合感染鸡的骨髓、脾脏、胸腺、法氏囊等免疫器官中淋巴细胞的凋亡进行了形态学研究，首次发现ALV-J可诱导胸腺、法氏囊和脾脏中不同程度的淋巴细胞凋亡，凋亡主要见于早期，ALV-J与REV混合感染时淋巴细胞凋亡尤为显著，以胸腺中最为明显。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

Methods The cultured 3T3 -L1 cells were pretreated with naringin, and then MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.

培养3T3-L1细胞，并用不同浓度的柚皮苷进行干预，以四甲基偶氮唑盐法检测细胞增殖，用油红O染色和染色比色法分析脂肪细胞的分化程度。

Specific stainings includ silver staining,Nissl bodystaining and modified trichrome staining with improvements.

组化染色包括嗜银染色、尼氏体染色及新的改良三色法染色。

The article mainly probes and researches the dyeing technology condition of polyester and cotton fabric with dispersing /reactive dye in one bath .

探讨了用分散/活性染料对涤/棉混纺织物进行一浴法染色的工艺条件，以及用载体法对涤/丝混纺织物进行一浴一步染色。

Cashmere products in dark shade are normally dyed with acid alizarine dyes, chrome content in fabrics is determined by fabric pretreatment with microwave digestion followed by atomic absorption spectrometer.

酸性媒介染料染深色羊绒是较为常用的染色方法，采用微波消解法对媒介黑PV染色羊绒制品进行预处理，再通过原子吸收光谱测定法测定样品的铬含量。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。