染色法

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

Results Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%. Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%; specificity was 88.9% and 94.4%; positive predictive value was 76.5% and 83.3% negative predictive value was 97% and 89.5%, using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%, positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.

Amsel法阳性率28%，组织多胺试验阳性率24%，一步法唾液酸酶活性的检测试验阳性率34%，革兰染色细菌评分法阳性率30%；唾液酸酶活性检测法与Amsel法，革兰染色细菌评分法相比较差异无显著性；组织多胺试验与Amsel法，革兰染色细菌评分法相比较差异无显著性；以Amsel法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%，特异性分别为88.9%和94.4%，阳性预期值分别为76.5%和83.3%，阴性预期值分别为97.0%和89.5%；以革兰染色细菌评分法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%，特异性分别为92.1%和94.3%，阳性预期值都为83.3%，阴性预期值分别为97.2%和86.8%。

For theimmunohistochemical double staining of MBP/NF,S-P method was used for bothstaining,with brown-yellow and purple-blue DAB for the first time and second timestaining.For the immunohistochemical double staining of CD31/IgM andGFAP/Ki-67,the first time staining was performed by S-P method with brown-yellow DAB,while the second time staining was performed by SABC-AP methodwith NBT/BCIP.

双重免疫组化染色包括：MBP/NF，两次染色均用S-P法，第一次显色为棕黄色DAB，第二次显色为紫蓝色DAB，以及CD31/IgM和GFAP/Ki-67，第一次染色用S-P法，棕黄色DAB显色，第二次染色用SABC-AP法，NBT/BCIP显色。

The specific binding ability of these IgY antibodies to SARS-CoV-infected cells was demonstrated by immunofluorescence assay.

在免疫萤光染色法中可见纯化的IgY与被SARS-CoV感染的细胞具有特异性结合反应。

Meanwhile ,we studied the pathogenesis of vocal fold polyp further.Methods: The expression of TGF-Bi and VEGF were detected in the examples of 37 vocal fold polys and 11 healthy vocal folds with immunohistochemical staining .

应用免疫组化SP染色法检测TGF-β_1、VEGF在37例声带息肉及11例正常人声带中的表达，并对其阳性表达结果进行比较。

Methods: Animal model of smoking was made and TTC staining method was used to detect infarct volume. The neurological function score and infarct volume ratio after focal cerebral ischemia in rats were detected.

方法建立吸烟大鼠模型，用TTC染色法测定梗死灶体积，检测大鼠局灶性脑缺血后神经功能缺陷评分及梗死灶体积占同侧半球百分比。

Of, relating to, or being a bacterium that retains the violet stain used in Gram's method .

革兰氏阳性的属于，有关或作为使用革兰氏染色法时保留紫色色素的细菌的

A positive test for pregnancy.Of, relating to, or being a bacterium that retains the violet stain used in Gram's method.

革兰氏阳性的属于，有关或作为使用革兰氏染色法时保留紫色色素的细菌的

Methods: BALB/c mice were intranasally infected with respiratory syncytal virus before or after sensitization to Japanese Cedar Pollen crude extract. Single-cell suspensions obtained from the lung parenchyma of inoculated mice were re-stimulated in vitro by co-cultivating with UV-RSV or JCP for 48 hr.

用花粉抽提物JCP致敏BALB/c鼠，致敏前或后经鼻感染RSV，提取肺组织细胞，体外培养，ELISA试剂盒检测培养上清中细胞因子水平；姬姆萨染色法分类并计数肺组织内浸润细胞数量；ELISA方法检测血清特异性抗体IgE水平。

Methods The expression of VEGF and MVD in 70 cases of breast diseases was examined by immunohistochemistry staining.

采用免疫组化染色法检测70例乳腺疾病患者（乳腺良性疾病患者28例，乳腺癌患者42例）术后标本中VEGF表达水平和MVD值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。