染色法

The variety 3 could be identified by phenol and guaiacol test, the other varieties also could be classified into three groups.

在快速化学鉴定方面，通过苯酚染色法和愈创木酚染色法，可以将品种3鉴定出来，并可将其它品种划分为三个组。

The stem was cut into several segments away from the pine seeding, arranged and collected in the order of chronology time and distance from where they were inoculated. To separate B.xylophilus coarsely out from the stems and made them into paraffin slices. And be observed under the potics microscope by dyed with safranin-fast green contrast-stain method and PI fluorescence stain method. In order to find out how about the nematode rigour distributing , but also ebb and flow of their amounts after invading the host pine seeding. Seek the pathological changing orderliness of host cells in different position along with the remotion of nematodes. By deeply analysing the variety of host cell nucleolus to estimate whether the host cell took place the programmed cell death after inoculated B.xylophilus.

按时间进程和与接种点上下距离的远近，对松苗分茎段进行粗分离和制作成石蜡切片，分别用番红-固绿双重染色法和碘化丙锭荧光染色法在光学显微镜下观察，以了解线虫侵染后在寄主体内的准确分布和数量的消长，寻找随着线虫的移动扩散，寄主不同部位细胞病变的数量、速度发生的变化规律，并进一步通过分析其细胞核的变化来判定松材线虫侵染后是否能诱发寄主细胞发生程序性死亡(programmed cell death，PCD)。

The effect of Alcian blue Safranin O staining was the best, which was applicable to identification of the mast cell of A. Japonica. The improved toluidine blue staining was not applicable.

AB/SO染色法效果最好，适用于日本鳗鲡肥大细胞的鉴定；改良甲苯胺蓝染色法不适合于日本鳗鲡肥大细胞的鉴定。

Methods The routine examination of serous membrane effusion are based on smearing and WrightGiemsa staining,the character of cells were evaluated by veteran in the field of cytodiagnosis.

浆膜腔积液常规检查的制片采用推片法，涂片染色采用瑞吉复合染色法，通过有经验的细胞学学者鉴定细胞性质。

Methods The routine examination of serous membrane effusion are based on smearing and Wright??Giemsa staining,the character of cells were evaluated by veteran in the field of cytodiagnosis.

浆膜腔积液常规检查的制片采用推片法，涂片染色采用瑞吉复合染色法，通过有经验的细胞学学者鉴定细胞性质。

Results: Average protein spots were 426 in the diarrhoea-predominant IBS group by using Coomassie brilliant blue staining, while the constipation-dominant IBS group by using silver staining got average 581 protein sptos.

结果：考马斯亮蓝法染色用于腹泻型IBS患者，平均得到蛋白质点426个；硝酸银染色法用于便秘型IBS患者，平均得到蛋白点581个。

Methods Stool specimens of 4 048 students at different educational levels were collected, oocysts of cryptosporidium were identified with auramine - phenol stain and improved ami - acid stain.

方法采集安徽省各地市不同教育层次学生粪便标本共4048份，采用金胺-酚染色法和改良抗酸染色法检查隐孢子虫卵囊。

A special staining method for the simultanouse demonstration of nodal cell, myordiac cell, collagenic and elastric fibers was applied on a same sheet of glass. This technique is simple and dired. There is contrast sharply with color.

本文建立了一种研究心脏传导系统和心血管形态学的特殊染色技术（改良的Masson's三色法十Verhoeff's弹力染色法），能在同一玻片上将起搏细胞、工作心肌、胶原纤维及弹力纤维区分开来，且操作简捷，色彩对比鲜明。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Same excrement specimen one the type be 2 and use iodine to dye a method respectively and improvement the anti- be sour dyeing method to carry on contrast test.

将同一粪便标本一式二份，分别用碘染色法和改良抗酸染色法进行对比测试。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。