染色法

Methods:The expression of mast cell tryptase and chymasec were examined in 37 cases of normal uterus with immunohistochemical double labeling techniques.

选取37例正常子宫组织，用双重免疫组化染色法检测肥大细胞中类糜蛋白酶和类胰蛋白酶的表达。

Is highly debated. Microscopic studies using DMACA staining revealed flavanols in shoot tip cells of Tsuga Canadensis during the early anaphase and in prophase nuclei.

用DMACA染色法的显微研究表明在在加拿大铁杉茎尖细胞分裂后期的早些阶段及细胞分裂早前期的细胞核中都有黄烷醇存在。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

The staining assays are fast and sensitive for determination of protein.

染色法一直是公认的快速而灵敏的蛋白质的测定方法，但在膜蛋白的测定中却显得无能为力。

This study re-evaluated the application of Gram's stain in diagnosing gonorrhea in women by comparing with gonococci culture.615 samples from 500 cases in clinic of Gynecology were studied.

本文用淋球菌培养比较研究对革兰氏染色法在女性淋病诊断中的应用作重新评价。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

Results the positive rates and specificity of fluorescence quantitative pcr, acid-fast stain,were100% and 30% respectively.

结果 荧光定量 pcr、抗酸染色法检测结核分枝杆菌的特异性分别为100%、30%。

Methods］ To examine feces with modified acid-fast staining, investigate the epidemic factor and the disease treatment.

方法］ 粪便用改良抗酸染色法检查，检出隐孢子虫者作流行因素调查与治疗。

Methods:Based on the model of Human umbilical vein endothelial cell,Rose Bengal Stain was used to study the influence of herbage containing serum of HLJDT on LPS or TNF-α induced neutrophil-endothelium adhesion by pretreated HUVEC4h、12h、24h.

中文摘要：以体外培养的人脐静脉内皮细胞为模型，应用蛋白染料染色法，观察HLJDT含药血清及其与LPSINF-α共同处理HUVEC4h、12h、24h后，对中性粒细胞与血管内皮细胞粘附的影响。

Double immunofluorescence staining revealed that the interaction of BRCA1 protein between cell plasm and nucleus stimulated BRCA1 nuclear export was obvious in different pancreatic tissues. BRCA1 nuclearcytoplasmic shuttling manner was correlated with cancer development.

免疫荧光双重染色法证实BRCA1蛋白在胰腺癌细胞胞质和胞核间互动，这是国内首次发现和报道BRCA1在胰腺癌组织细胞中胞质和胞核间互动现象和其亚细胞分布情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。