染色法

The models of EIOP were established by obstructing episcleral veins, using immunohistochemistry stain for PEDF expression in retina and evaluate the rule of the change.

应用巩膜浅层静脉烧烙法制作慢性高眼压(elevated intraocular pressure， EIOP)模型，用免疫组织化学染色法观察视网膜各层结构PEDF的表达和变化规律。

Fibrillar collagen(Collagen typeⅠand Ⅲ) in sinusoids of normal liver was successfully observed through second harmonic generation microscopy.

分别用前向二次谐波及背向双光子荧光对肝脏标本的成纤维胶原和肝细胞浆进行成像，将结果与传统的Masson's三染色法进行比较。

Methods The expressions of Rb protein were detected in 52 patients with breast cancer, 20 patients with breast fibroadenoma or breast cystic hyperplasia by S-P immunohistochemical method.

采用S-P免疫组织化学染色法对52例乳腺癌组织和20例良性乳腺病变组织中Rb蛋白的表达进行检测。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

We observed testes, epididymis, ovaries, oviducts and uteri in six species of microbats by the Haematoxylin and Eosin staining of frozen tissue sections and found that sperm storage in five of the 6 species.

采用冰冻切片和苏木精－伊红染色法，观察了6种蝙蝠的睾丸、附睾、卵巢、输卵管和子宫，发现5种蝙蝠有精子储存现象。

The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.

应用组织化学染色法和聚合酶链反应技术对口腔白斑是否存在念珠菌进行检测。

With the methods of molecular biology, in situ hybridization, immunohistochemistry, immunohistochemistry, radioimmunodetection, Electrophysiology, we have studied the depressant effect for hypermyotonia of experimental cerebral infarction rat and its the gamma-aminobutyric acid receptor mechanisms first time when we simulated the GB 34 with electric acupuncture.

本课题以大鼠实验性脑梗死动物模型为研究对象，采用分子生物学原位杂交技术、免疫组织化学染色法、放射免疫检测技术、神经电生理记录方法，首次较系统研究了电针阳陵泉对脑卒中动物肢体肌张力增高的抑制作用及其GABA受体机制。

Methods The expression of p15 mRNA and p15 protein was examined respectively by in suit hybridyzation and streptavidin-horseradish peroxidase immunohistochemistry method in 48 cases of esophageal carcinoma, 18 cases of esophageal dysplasia and 10 cases of incisal margin normal tissues.

采用原位杂交技术和链霉卵白素－过氧化物酶(streptavidin-horseradish peroxidase， SP)免疫组织化学染色法检测48例食管鳞状细胞癌组织，18例癌旁不典型增生组织和10例切缘正常组织中p15mRNA及其蛋白的表达水平。

Leucocytic density in semen was detected by peroxidase staining.

采用过氧化物酶染色法检测精液中白细胞密度。

Our immunostaining data showed that LRH-1 were present in the nucleus of large and small luteal cells.

以免疫细胞染色法对初代培养细胞进行分析发现，LRH-1同时存在大小两种黄体细胞的细胞核中。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。