染色法

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠随机分3组：糖尿病组、高蛋氨酸组和叶酸、维生素B12组，C57BL/6鼠为对照(C57)，以相应饲料喂养4个月后取材，测定血糖、血同型半胱氨酸、叶酸和维生素B12，以PAS、六氨银染色法光镜下及超微电镜观察各组DN病变，比较各组肾小球硬化指数；以免疫组化和半定量图像分析肾小球MMP-9蛋白表达，并以RT-PCR检测肾脏MMP-9 mRNA水平。

Methods Immunohistochemistry of streptavidin-perosidase method was applied in the observation of the expression of TIAF-1 in each group, including tissues obtained from the unaffected part of nephrectomized kidneys with renal cell carcinoma (n=6) and cadaveric donor of normal kidneys (n=8) as the control group, tissues with acute rejection (AR, n=16) and those with chronic rejection (CR, n=28), and in the analysis combined with the number of positive cells of CD3, CD20 and CD68 infiltrated in the interstitium.

采用SP免疫组织化学染色法对6例因肾细胞癌行肾切除的未受肿瘤侵犯的正常肾组织和8例供肾正常肾组织、16例急性排斥反应和28例慢性排斥反应移植肾组织中TIAF-1的表达进行观察，并对间质浸润细胞中CD3、CD20、CD68阳性细胞数进行分析。

The average neuronal neurite length and the percentage of differentiated neurons were detected by immunocytochemistry staining.

免疫荧光染色法观察各种亚型神经细胞的比例并比较神经元样细胞的轴突长度。

Methods:The expressions of TGF-β1 and TGF-β2 were examined in surgical specimens of 84 patients with lung carci noma by SABC immunohistochemistry.

用SABC免疫组化染色法检测84例肺癌组织标本中TGF-β的表达。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上，於是我们将SPANXA1转染在CL1-5细胞株中，并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力，探讨SPANXA1对癌细胞性状的影响，结果发现增加SPANXA1会降低癌细胞的转移能力，在另一方面，我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中，并以西方点墨法再次证实SPANXA1是存在於核质中。

Objective:To introduce a fast diagnostic way of gonococcal ophthalmia in newborn,ie,TF staining method.

目的：探讨一种快速诊断新生儿淋菌性眼炎的方法——TF染色法。

Combined with the related illness of parents(especially illegimate sexual act),the TF staining method could rapidly examine gonococcus in eye secretion of newborn.So it is suggested that the TF staining method possessed a stronger utilized value in the diagnosis of gonococcal ophthalmia in newborn.

TF染色法可快速检查新生儿眼分泌物中的淋球菌，同时结合其家长的病史对快速诊断新生儿淋菌眼炎具有较强的实用价值。

To analyze WWP1 protein expression in clinical samples, we applied IHC technology to detect the level of WWP1 protein in 18 paired OSCC samples, our data represented different they had different protein pattern and location.

应用免疫组织染色法侦测18对成对口腔癌样本WWP1蛋白表现，发现不同的样本呈现不同的蛋白表现量，表现在细胞的位置也有所不同。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）有较好的抑制生长作用，ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml，对肝癌细胞hepg2抑制作用差， ic50为0.937 9 mg/ml；白花丹石油醚提取部位对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml；乙酸乙酯提取物对肺癌细胞（lu-04）有一定的抑制作用，ic50为0.734 3 mg/ml；水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好，值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

The assay has better sensitivity, producibility, no isotope contamination. It is simpler and more rapid than silver stain protocol.

该法有较好的测定敏感度与重复性，较银染色法更为简便快速，无同位素污染，检测成本低，值得临床推广

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。