染色法

Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。

Based on the four-vessel occlusion models, neuron apoptosis in CA1 region of hippocampus was detected by cresyl violet staining, the phosphorylation of JNK and Bad (ser128) were detected by immunoblot...

以大鼠四动脉结扎脑缺血模型为基础，应用焦油紫染色法检测海马CA1区神经元凋亡，采用免疫印迹检测JNK和Bad(ser128)的磷酸化。

Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.results it was found that hpk1 was expressed in rat hippocampus neurons.conclusions administration of hpk1 antisense oligodeoxynucleotides can significantly protect hippocampus neurons from apoptosis without dose-dependence.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。结果在大鼠海马神经元中有hpk1的表达，脑室注射as-odns大鼠海马ca1区存活锥体细胞明显增多。结论 hpk1反义寡核苷酸对缺血/再灌注引起的海马神经元凋亡具有保护作用。

Learning and memory performance of rats was measured by using Morris water maze first and Y-maze afterwards. Neurons in the dentate gyrus, CA3 and CA1 regions of the hippocampus were stained by using Cresyl violet method and counted.

采用Morris 水迷宫和Y-迷宫测试大鼠学习与记忆成绩，并用Cresyl violet染色法对大鼠海马结构进行神经细胞计数。

Basing on the four-vessel occlusion models,neuron apoptosis in CA1 region of hippocampus was detected by cresyl violet staining,the phosphorylation of Akt and Bad(ser136)were detected by immunoblotting.

以大鼠四肢动脉结扎脑缺血模型为基础，应用焦油紫染色法检测海马CA1区神经元凋亡，采用免疫印迹检测Akt和Bad(ser136)的磷酸化。

Cytotoxicity of TNF in immortalized lymphoblastoid cell lines was determined by crystal violet staining.

应用结晶紫染色法检测淋巴母细胞系分泌TNF的细胞毒活性。

METHODS: Cytotoxicity of the combination of TNF-α and IFN-γ on HCC in vitro was measured by using a crystal violet staining method.

采用结晶紫染色法测定肿瘤坏死因子-α、干扰素-γ和两者联合用药体外杀灭肝癌细胞的能力。

Methods Add LHRH and LHRH-PE40 into Hela cell culture, stain with crystal violet and determine the A value.

在Hela细胞培养液中，分别加入LHRH和LHRH-PE40，经结晶紫染色法检测A值。

Methods:Using cell culture technique,the inhibitory effects of NCTD on 3AO and AO cells were measured by way of crystal violet dyeing.The alterations of cell cycle induced by NCTD were analyzed with flow cytometry.

利用细胞培养技术，以结晶紫染色法测定NCTD对卵巢癌3AO和AO细胞的抑制作用，并利用流式细胞仪测定分析NCTD作用下两种肿瘤细胞的细胞周期变化。

Methods Inhibitory effects of NCTD on 3AO and AO cells in vitro were measured by crystal violet dyeing;morphological and ultrastructural changes were observed by light and electron microscopes.

利用细胞培养技术，以结晶紫染色法测定NCTD对3AO和AO细胞的体外杀伤作用，并在光镜和电镜下观察NCTD对其进行治疗，以观察NCTD体内对卵巢癌的抑癌效应。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。