染色法

It was dose-dependent. Apoptotic cells were observed by TEM and FCM.4 By H-E, we observed acidophilic stain of cells.

流式细胞技术、电镜下可观察到明显出现的凋亡细胞； 4通过苏木精一伊红染色法，观察到处理后细胞出现出现嗜酸性的浓染。

The experiment material is apricot and almond in this paper,passed TTC method to carried on examination the agnate pollen of vitality before pollination;after the investigation fruit set percentage,using of DPSv3.01 analyzed the date;the pollen tube behavior and bourgeon were observed by means of flurescence microscopy;the orthogonal design was used to optimize SSR amplification system of apricot hybrids in five factors at four levels respectively;making use of the new SSR-PCR system analyse the 50s hybrids.

本文以仁用杏及扁桃为试验材料，通过TTC染色法对授粉前父本花粉的活力进行了测定，运用DPSv3.01专业版数据处理系统软件对授粉后坐果率的调查数据进行了分析；利用荧光显微技术对花粉在柱头的萌发情况及花粉管的生长状况进行了动态观察；采用正交设计对仁用杏杂交苗SSR-PCR反应的5因素4水平进行试验；在新体系的基础上对50个杂交苗进行了分析。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Gram,Hans Christian Joachim(1853—1938) Danish bacteriologist. He devised the important method of staining bacteria that bears his name (see Gram ' s stain) in Berlin in 1884, building on the method of Paul Erlich.

Gram，Hans Christian Joachim(1853—1938)：丹麦细菌学家，他于1884年基于 Paul Erlich 的方法在柏林发明了一种重要的细菌染色法，并以自己的名字命名之（参见 Gram ' s stain ）。

Relative root elongation, Al content and callose content in root tips and hematoxylin staining were compared in evaluating plants' tolerance of Al stress.

利用相对根伸长率、根尖Al含量和根尖胼胝质含量等三项参数结合苏木精染色法，研究Al胁迫下，各参数在评价植物耐Al性上的地位，以及各参数间的相互关系。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

after treated with glu (5 mmol/l) for 24 h, the viability of pc12 cells depressed and the viable cells was only 58.3%when compared with cell control group tested by mtt assay.

体外培养pc12细胞，建立谷氨酸诱导pc12细胞损伤的模型；采用mtt法检测细胞活力，流式细胞术和hoechst 33258 dna 染色法检测细胞凋亡。

METHODS The killing effects of NDV-L on cancer cells and normal cell were observed by means of plaque assays, HA titer test and MTT test. The inducing effects of NDV-L on apoptosis were observed by means of Giemsa's dying test, phase contrast light microscopy, transmission electron microscope, DNA extraction, and flow cytometry.

通过噬斑形成单位、HA效价、MTT法等方法检测NDV -L对细胞的杀伤作用；通过Giemsa's染色法、倒置相差显微镜、透射电镜、DNA凝胶电泳、流式细胞仪检测NDV -L诱导肿瘤细胞凋亡的作用。

Methods The specimens from the acquired middle ear cholesteatoma tissue of 22 cases and the normal external ear skin of 10 cases were examined by immunohistochemical S-P method and computer image analysis.

应用免疫组化SP染色法和计算机图像分析法，检测22例胆脂瘤上皮和10例正常外耳道皮肤中Ras蛋白的表达情况。

The result of immunohistochemical staining was different from that of conventional Congo red Staining.

βA4在脑组织中的表达并非Alzheimer病所特有，而是多种神经变性疾病的共同特征；βA4免疫组化法的阳性结果不同于传统的刚果红染色法。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐