染色性

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞，P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%，20%，50%，80%，100%)的胶原/透明质酸支架中，在无血清培养液中三维培养。2周后，用SO染色和免疫组织化学染色分析，观察硫酸软骨素和Ⅱ型胶原合成情况。

Meiotic pachytene bivalents were obtained from porcine testes using prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution. Mitotic metaphase chromosomes were prepared from blood cell culture. Comparative studies on division index and length of pachytene bivalents and mitosis metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87～5.98) times longer than the latter, respectively. Chromomere maps of bivalents are more abundant than mitotic metaphase G-bands, while they are correspondent with mitotic early-metaphase G-bands. The result was found by using the chromosome 12 as a sample.

以性成熟公猪睾丸和外周血为材料，采用长低渗、高氯仿卡诺固定液固定和外周血细胞培养制备减数分裂粗线期二价体和有丝分裂中期染色体，通过对二价体和有丝分裂中期染色体分裂指数和长度的比较研究，发现二价体的分裂指数和长度分别是有丝分裂中期染色体的5倍和3.42倍(1.87～5.98)；同时以12号染色体为例，比较了二价体上的染色粒结构带与有丝分裂中期染色体G-带，表明染色粒结构带比中期染色体G-带带纹丰富，而与早中期G-带带纹吻合。

A SSR marker based linkage map covering chromosome 10 and the short arms of chromosomes 1 and 11 of rice was constructed and applied for mapping fertility-restoring genes in Zhenshan 97A/(Zhenshan 97B/Milyang 46)F_ 6 population consisting of 704 lines.

应用由704个株系组成的珍汕97A/(珍汕97B/密阳46)F6测交群体，针对水稻第10染色体和第1、11染色体短臂构建了微卫星标记连锁图谱，检测到控制野败型细胞质雄性不育育性恢复的4个QTL，其中位于第10染色体长臂中下部的Rf4具有主效效应，位于第1染色体短臂的Rf3具有较大效应，位于第10染色体长臂近着丝粒处的qRf10和第11染色体短臂近着丝粒处的qRf11表现出微效作用。

Methods Bioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

MethodsBioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

Results:The main manifestation of A group was transient hepatocyte hydropic degeneration; in group B the degeneration of hepatocytes was most severe in the 2nd week. Fibrosis appeared in the 4th week,and obvious pseudolobules were formed in some parts of the liver in the 6th week.

结果：A组HE染色肝细胞呈一过性水样变，变性在2周时最重，至6周已明显减轻。B组HE染色2周时肝细胞明显水样变，4周时可见汇管区纤维组织增生，6周时部分肝组织出现明显假小叶结构。

Compared the similarity and difference of distribution of homologous fragments on X and Y, we suggested that the sex chromosomes of spiny eel have evolved from a pair of homologous ancestral chromosomes, and during the evolution, a pericentric inversion might have occurred on the original Y chromosome, and subsequently terminal regional duplication might have followed, eventually resulting in the differentiation and formation of X and Y chromosomes, then the recombinants between sex chromosomes were repressed, so the sex chromosomes could be inherited steadily.

根据X、Y染色体上同源片段的分布的相似和相异，我们对刺鳅性染色体的分化历程进行了推测，即：刺鳅的性染色体起源于一对原始的同源染色体，通过原Y染色体上的一个明显的臂间倒位，以及染色体末端的异染色质成分的扩增，导致原始Y染色体与X染色体在染色体结构上的分化，从而抑制了X、Y性染色体之间的遗传物质的重组交换，为性染色体的稳定遗传奠定了基础。

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性，我们还给23例睾丸的非GCTs（10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤）和275例非睾丸性肿瘤（158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤）做了SALL4免疫染色。

ObjectiveTo study the effects of a natural herb A on the monocular form-deprived and strabismic cat s with the technique of PVEP、 SVEP and single cell unit recording.

研究目的采用图形视觉诱发电位、扫描视觉诱发电位和细胞外记录技术研究天然药物A对形觉剥夺性和斜视性弱视猫视皮层机能的影响；应用HE、尼氏染色和透射电镜观察天然药物A对形觉剥夺性和斜视性弱视猫视皮层形态学的改变。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。