染色性

Flowers yellowish to greenish, rarely brightly colored, small, bisexual or unisexual, rarely polygamous,(4 or)5-merous, hypogynous to epigynous, in mostly axillary, sessile or pedunculate cymes, or reduced to few in fascicles. Calyx tube patelliform or hemispherical to tubular, sometimes absent, at rim with calyx, corolla, and stamens; sepals 4 or 5, valvate in bud, triangular, erect or ± recurved during anthesis, adaxially often distinctly keeled, alternate with petals.

对带绿色淡黄的花，很少明亮染色，单性，很少杂性的小，两性或，(4或)5瓣，萼筒小盘状或者半球形到管状，有时无，在凸缘有花萼，花冠和雄蕊；萼片4或5，芽期镊合状，三角形，直立或者在花期弯曲，正面通常显著凸起，与花瓣互生。

The intracellular expression of viral gene and phenotypical change of transformants were examined.

结果：分离获得了可长期体外培养的SV40转化细胞克隆，已传23代以上，体外存活超过250d；DNA印迹实验显示SV40大T基因已整合至细胞染色体内，间接免疫荧光检测示多数细胞胞核内有大T基因产物的表达；转化细胞对裸鼠无致瘤性，并可正常表达角蛋白。

Methods The positive stained area was compared between 22 STC cases and 12 non obstructed colon cancer cases using immunohistochemistry technique and pathology micrograph analysis system with 100 multiple photics microscope.

方法应用免疫组织化学技术和病理显微镜分析图像系统，在中倍光镜下计算22例STC患者和12例非梗阻性结直肠癌患者的阳性染色区域的面积并进行对比。

The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The ultrastructural changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 postinfection,the apoptosis cells shrank,the cytoplasm concentrated,the chromatin condensed,the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 postinfection,the apoptosis cells evidently shrank,the cell membranes protu berated,the plastosome manifolded,and apoptosis bodies were found. During the last stage from day 10 upwards postinfection,the apoptosis bodies degenerated and disappeared,and no inflammation occurred. Few apoptosis cells were necrotic.

结果表明，PRRSV可诱导宿主肺和子宫发生典型的细胞凋亡，表现为细胞的超微形态结构随着病毒感染进程出现不同的特征性变化，早期（感染后第8 h至第3 d）凋亡细胞多表现为细胞体积缩小，细胞质密度增强，染色质浓缩，核仁解体，内质网扩张；中期（感染后第5 d至第9 d）凋亡细胞体积显著缩小，细胞膜突起，线粒体增多，有凋亡小体形成；晚期（感染后第10 d以后）凋亡细胞形态多表现为凋亡小体降解和消失，少数凋亡细胞在凋亡晚期表现为坏死细胞。

A study was carried out in this paper on the dyeing and finishing performance of a water-soluble sulfur black dye containing polycarboxylic groups.

对合成的含多羧基水溶性硫化黑的染色和防皱整理双功能性进行了研究。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

To investigate the possibility of such a transition, we studied 17 cases of MGA or atypical MGA some of which had given rise to carcinoma in situ and/or invasive ductal carcinoma using the reticulin stain, immunohistochemistry (S-100, p63, Ki-67, and p53), and a molecular approach involving microdissection and high-resolution comparative genomic hybridization and MYC chromogenic in situ hybridization.

为了研究这种转变的可能性，我们分析了17例典型或非典型的MGA，其中一些已经发展成为原位癌和/或浸润性导管癌，所用的方法是网状染色、免疫组化（S-100， p63， Ki-67， and p53）和分子技术，分子技术包括显微切割和高分辨率比较基因组杂交及MYC显色的原位杂交。

Immunohistolochemical stain by monoclonal antibodies for growth factors and actin was used to detect whether there are abnormal expressions in saccular aneurysms.

利用血小板源性生长因子、转化生长因子（TGF-β1）、血管内皮细胞生长因子和肌动蛋白的单克隆抗体进行免疫组织化学染色。

The Fathers call Mary the tabernacle exempt from defilement and corruption (Hippolytus,"Ontt. in illud, Dominus pascit me"); Origen calls her worthy of God, immaculate of the immaculate, most complete sanctity, perfect justice, neither deceived by the persuasion of the serpent, nor infected with his poisonous breathings ("Hom. i in diversa"); Ambrose says she is incorrupt, a virgin immune through grace from every stain of sin Sermo xxii in Ps.

cxviii；父亲呼吁玛丽帐幕免受污辱和腐败（ hippolytus ，& ontt在通谕，上主对我科技局首席助理秘书长&）；呼吁渊源，她无愧于上帝，圣母无染原罪的圣母无染原罪，最完整的神圣性，完美的正义，既不是欺骗，劝说该蛇，也感染了他的毒breathings （&磡，我在diversa &）；刘汉铨说，她是廉，处女免疫透过恩典，从每一个染色的单仲偕（ sermo二十二，在

The method combined fluorogold retrograde with fluorescent immunohisto chemistry was used to study c-fos expression of the neurons of brian stem proje cting to the subnucleus reticularis dorsalis under noxious stimulation.

用荧光金逆行追踪和FOS荧光免疫组织化学染色相结合的方法对外周伤害性刺激下大鼠脑干内向延髓网状背侧亚核传入投射的神经元中c-fos的表达进行了研究。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐