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So we selected the colchicine optimumparameters were 0.2μg/ml and 6 hour cultured duration.The third, there were significantly differences percent between 10 hourgroups and other three groups (4 hour groups, 6 hour groups and 8 hour groups) in0.1μg/ml groups; and there were significantly differences between 8 hour groupsand 10 hour groups, but they had no significantly differences among other twogroups in 0.3μg/ml groups; and there were significantly differences between 10hour groups and 6 hour groups, but 10 hour groups had no significantlydifferences among other two groups in 0.5μg/ml groups; and there weresignificantly differences between 8 hour groups and other three groups in0.7μg/ml groups on the metaphases (P<0.05), in the experiment of the effect onthe mouse 4- cell embryo stage single blastomere of vinblastine with differentconcentrations and duration, by x~2 statistical analysis.

根据实验的实际情况,适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.2μg/ml和6小时。3、在确定长春花碱不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中,经统计分析,阻断培养0.1μg/ml浓度组中10小时组与其他三组差异显著(4小时组、6小时组和8小时组);阻断培养0.3μg/ml浓度组中8小时组和10小时组间差异不显著,但与其他两组差异显著;阻断培养0.5μg/ml浓度组中10小时组与6小时组差异不显著,而与其他两组差异显著;阻断培养0.7μg/ml浓度组中8小时组与其他三组差异显著(P<0.05)。

Sowe selected the vinblastine optimum parameters were 0.1μg/ml and 8 hourcultured duration.The fifth, there were significantly differences between 0.45% sodium chloride groups and other two groups (0.5% sodium citrate groups and 0.35%potassium chloride groups) on the metaphases (P<0.05), in the experiment of theeffect on the mouse 4-and 8-cell embryo stage single blastomere of differenthypotonic fluids, by x~2 statistical analysis.

根据实验的实际情况,适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg/ml和10小时。5、在确定不同种类低渗液处理对小鼠4、8-细胞期胚胎单卵裂球中期分裂相数影响的实验中,经统计分析,0.45%氯化钠低渗液组与其他两组(0.5%柠檬酸钠和0.35%氯化钾)差异显著(P<0.05)。

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