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染色体数

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The F2 nullisomic amphidiploid with low chromosome number (2n=44-49) wasaccompanied with abnormal meiosis (7.45 Ⅰ+ 19.50 Ⅱ), but its fertility (19.2%) wasmuch higher than 〓's.

F2非整倍体的染色体数较低(2n=44-49)。

Having a chromosome number that is not an exact multiple of the haploid number .

染色体数不是单倍体的整数倍。

The chromosome number of these two lines is 2n=34, which is the same as H.

观察其根尖生长点切片,发现染色体数为2n=34。

Centromeres could be identified easily on the bivalents at diplotene, so it was convenient to analyse the karyotype.

结果表明,日本沼虾染色体数n=52,2n=104,据双线期二价体的相对长度、着丝点位置以及形态诸方面的特点分析了核型,共分为A,B,C,D4个染色体组,核型组成是N=37M+4ST+11T;初级精母细胞减数分裂前期I可分为细线期/偶线期、粗线期、双线期和终变期5个时期,双线期的二价体存在弥散阶段,核仁明显,且双线期二价体着丝点清晰,给核型分析带来了方便。

Result It was found in the results that the better pretreatment time for onion root-tip was 3.0 h and its better dyeing solution was basic fuchsin dyeing solution. On this basis, it was found that the chromosome number of tested onion was 2n=2x=16 through chromosome karyotype analysis on the root-tip cells of onion.

结果]结果发现,洋葱根尖的较好的预处理时间为3.0 h,较好的染液为碱性品红染液;在此基础上,通过洋葱根尖细胞染色体核型分析,发现试验所用洋葱的染色体数为2n=2x=16。

The analysis result indicates that the chromosome number of 38 accessions is ranged from 63 to 90 as same as septuploid, octoploid, enneaploid and decaploid, respective.

结果发现台湾鱼腥草收集品染色体数目介於63~90条之间,分别为七、八、九及十倍体,另发现染色体数84与85条之异数体收集品。

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明,茎尖分生组织培养的幼苗及丛生苗遗传稳定,其染色体未发生倍性变异,均为2n=16;愈伤组织及其再生苗遗传稳定性较差,愈伤组织染色体数变异率为43.4%,其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%;愈伤组织分化苗染色体变异率为11.7%,其中单倍体占6.7%,三倍体占1.7%,四倍体占3.3%。

Orientale additional and substitutional lines. chromosome number of 96 BC2 F3 individuals were accounted. Fifteen plants with 2n= 43 and eight plants with 2n= 44 were checked out. GISH results of the individuals with 43 chromosomes showed that they blonged to three different monosomic additions. Two disomic additions, ine double-monosomic addition, one trible- monosomic addition and one monosomic addition were found in the 2n = 44 individuals. One double-monosomic substitution and one monosomic sustitution were obtained.

为了筛选小麦-旱麦草异附加系、异代换系,对普通小麦品种Fukuho×东方旱麦草属间杂种的BC2F3代材料的96粒种子进行了染色体数目的检测,共检出15粒2n=43的种子,8粒2n=44的种子,进一步对以上材料进行的基因组DNA原位杂交,共鉴定出3个单体附加系,2个二体附加系,1个双单体附加,1个小麦三体单体附加,1个附加3条东方旱麦草染色体的小麦单体,在染色体数为42的个体中,检测出1个单体代换,1个双单体代换。

Somaclonal variations analysis on mixoploid hybrid plant BI-1 showed that percentage of cells with 2n=25, 26 decreased and percentage of cells with 2n=29 ascended.Chromosome segments of I.

并对混倍体杂种BI-1的无性系变异进行了分析,发现染色体数目为25、26的细胞比例呈明显下降趋势,而染色体数为29的细胞比例明显上升,其他染色体数目的细胞比例处于动态变化之中。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象,对其进行常规正反杂交后子房的离体培养,通过离体子房培养获得的杂种种子经MS培养基诱导成苗,获得了芥菜型油菜与白菜的杂种F1代植株,表现出杂种优势;通过细胞学的方法,鉴定了杂种F1幼蕾体的细胞染色体数为2n=28,为母本芥菜型油菜(2n=36)和父本白菜(2n=20)的单倍体染色体数之和;芥菜型油菜与白菜常规杂交后,经离体子房培养获得了&胚性芽&,确立了植物胚挽救的另一种形式。

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