染色体基质

RESULTS: ADMSCs at passage 3 were positive for CD13, CD59 and CD44, but negative for CD34 and HLA-DR. Chromosomal morphous, structure and number were normal, showing diplont structure. Under the microscope, there were complete Golgi complex, plenty of rough endoplasmic reticulum, and some glycogen granules in cytoplasm.

结果：第3代脂肪间充质干细胞CD13，CD44，CD59均呈阳性表达，而造血干细胞表面标志CD34及成纤维细胞表面标志HLA-DR均呈阴性表达；染色体的形态、结构和数目均未见异常，呈二倍体结构；镜下可见完整的高尔基复合体，数量较多的粗面内质网，部分糖元颗粒分布在胞浆中。

Here we describe 55.8 million base pairs of highly accurate finished sequence representing 99.9% of the euchromatin portion of the chromosome.

作者描述了5.58千万碱基序列，涵盖了该染色体常染色质99.9％的精确序列。

It is well-known that for eucaryotic cells, DNA is located in the karyon and can not penetrate to the cytochylema and the tissue fluid because the cell membrane can act as barriers for DNA transportation. Therefore, the appearance of DNA in cell matrix and the serum can indicate that some diseases have taken place. A main reason for cancer occurrence is the mutation of DNA. It may cause the change of the sequence and expression of cancer suppressor genes.

真核细胞的DNA广泛的存在于细胞核内的染色体上，由于核膜的选择性通透作用，使DNA无法分散到其它细胞的亚器官，加之细胞膜的隔离效果，DNA更难扩散到细胞外基质中，因此细胞外基质中DNA含量的增加往往也会预示某些疾病的发生。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

To address this question in an unbiased and genome-wide manner, we developed a new method, methylation-specific digital karyotyping, and applied it to epithelial and myoepithelial cells, stromal fibroblasts from normal breast tissue, and in situ and invasive breast carcinomas.

为了能无偏向地并在基因组范围解答这个问题，我们开发了一种新的方法——甲基化特异性数字染色体组型，并将这一方法应用于上皮细胞和肌肉上皮细胞、正常乳腺组织来源的基质成纤维原细胞、原位及侵入性乳腺癌细胞。

MAIN OUTCOME MEASURES : Culture of bone marrow stromal cells and spermatogonial stem cells and coloration and count of chromosomes.

主要观察指标：骨髓基质及精原干细胞的培养及染色体显色与计数。

By the method of SDS-PAGE, the main proteins of nuclear, matrix in salivary gland were proteins of 67kd, 66kd, 43kd, 38kd and 35kd Comparing the NHP composition of the nuclear matrix to that of the polytene chromosome, it can be seen that the nuclear matrix and the polytene chromosome shared many common NHP components, such as 43kd, 38kd and 35kd proteins.

通过SDS-聚丙烯酰胺凝胶电泳看到，果蝇唾腺细胞核基质的主要蛋白成份为67kd、66kd、43kd、38kd及35kd等多肽。与多线染色体骨架的蛋白成份比较，发现它们共同具有43kd、38kd及35kd等蛋白质。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。