染色体基因

In this study,we have identified C2H2 ZFP genes in the silkworm,and acquired the basic information for these genes,such as gene distribution on chromosome,gene family information and expression information.This information will be useful for further functional studies on these genes.

综上所述，本研究对家蚕基因组中的C2H2型锌指蛋白基因进行了鉴定，并通过分析获得了这些基因的基本信息，即染色体分布、基因家族信息和基因表达信息等，这些信息为进一步对家蚕C2H2型锌指蛋白基因进行功能研究打下了基础。

The common features of imprinted genes are, clustering of multiple imprinted genes in one chromosomal region (around 80%), conservation of imprinting among eutherian mammals, asynchrony of DNA replication of imprinted genes, temporal and spatial regulation of expression of imprinted genes, coding for untranslated RNAs as well as proteins, antisense transcripts may regulate expression of imprinted genes. Once established, the imprinting pattern is stably transmitted through cell division but reset in germ cells of the fetal gonads.

约80%的印记基因呈串出现在染色体上；在哺乳动物品种之间，印记基因具有较高的保守性；印记基因的复制通常表现为不同时性；一些印记基因具有印记遗传的时空性；少数印记基因只转录为mRNA而不翻译成蛋白质；印记基因的反意链通常表达，表达产生具有调节印记基因的作用。

Compared to japonica type parent, indica type parent (Taichung-Sen 10) contributed more genetic materials in the chromosomes 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, and 12, to the RIL population, but less in the chromosome 7. The averaged homozygosity (94.6％) was deviated from the expected ratio 96.9％.

十二对染色体之平均同结合与异结合比例分别为94.6％及5.4％，偏离F6世代同结合基因型之比例理论值(96.9％)，而分别在第六对染色体上有最大之同结合比例(98％)，第二对染色体有最小之同结合比例(90％)。

Methylation is negative correlation with genetic transcription , and the mechanism is probably as follows : directly interfere the combination of specific transcripton and recognition site of each promoter ; mediated transcription inhibition by change the construction of chromatin; induced gene silencing by combination of methylation site and transcription inhibition. P16 gene is also called multiple tumor supresser gene , which is the best easily to methylate in the anti-oncogenes. P16 gene locates in 9p21 of mankind-chromatosome , and it's total length is 8.5kb .

甲基化与基因转录呈负相关，其机制可能是[3]：直接干扰特异的转录子和各自启动子识别位点的结合；通过改变染色质结构，介导转录抑制；甲基化DNA位点直接与转录抑制子结合，诱导基因沉默。p16基因又称为多肿瘤抑制基因( multiple tumor suppressor 1 ，MTS1) [4] ，是肺癌中最易发生甲基化的抑癌基因，该基因位于人类染色体9p21上，全长8.5kb ，由两个内含子和三个外显子组成。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

And the research materials were often meiotic pachytene chromosomes of sex gland and mitotic prometaphase of early embryos and diapause eggs\' somatic cells.

早期，家蚕染色体识别和基因定位研究主要利用性腺减数分裂粗线期的染色体、早期胚胎和滞育卵有丝分裂早中期的染色体作为材料，按照相对长度和部分限性易位系统或缺失等来进行核型分析。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

It was found that one of the fragments was similar to the Peroxidase gene located on the rice chromosome 1 completely, another fragment was partially similar to the protein Phosphorylase gene located on the rice chromosome 12 with 99.99% homology.

同源性均在99％以上。其中一个与位于水稻第一号染色体的过氧化物酶基因的部分片段有100％相似性。另一个与位于第十二号染色体上的蛋白磷酸化酶基因有99.99％的同源性。

The break on chromosome 9 involves a gene called ABL. The break on chromosome 22 involves a gene called BCR.

9号染色体的断裂发生在ABL基因；22号染色体的断裂发生在BCR基因。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。