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A micronuclear version of the GARP gene was amplified by polymerase chain reaction. The macronuclear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuclear chromosomes of hypotrichous ciliates.

大核中该基因的染色体全长460 bp,基因两端具有下毛类纤毛虫大核特有的端粒序列(C4A4C4A4C4A4C4),开放读框内含有一个TGA(88-99)密码子,在游仆虫中编码为半胱氨酸。

Objective: To study the source of the separation of our hospitals to methicillin-resistant Staphylococcus aureus in molecular epidemiology of the characteristics, identify MRSA clinical isolates in our country, investigate the distribution characteristics of staphylococcal chromosomal cassette mec typing of MRSA and detect the Panton-Valentine leukocidin virulence gene of MRSA.

目的:研究我国医院来源分离到的耐甲氧西林金黄色葡萄球菌的分子流行病学特征,鉴定我国临床分离的MRSA,分析MRSA葡萄球菌染色体mec盒基因型,检测MRSA的PVL毒力基因。

In order to reveal clearly the genetic character of the dwarfing polish wheat, artificial crossing, cytological and morphological studies were carried out in present study. In addition, the chromosomal location of dwarfing and long glume character of dwarfing polish wheat was studied by means of Langdon monosomic analysis.

本试验:(1)通过矮秆波兰小麦与8个来源不同或染色体组组成不同的小麦属材料杂交,对杂种F_1进行细胞遗传学分析;(2)采用单体分析法,对矮秆波兰小麦的矮秆、长颖基因进行了基因定位。

Comparative analyses show a fascinating picture of conservation and divergence, revealing large blocks of gene orthology with rodents, scattered regions with more recent gene family expansions and deletions, and segments of coding and non-coding conservation with the distant fish species Takifugu.

比较分析表现出一副保守和分化的有意思的图片,一方面染色体中展示了一大组与啮齿类动物同源的基因,还有更多新近基因家族扩张和缺失的散步区域以及和进化上遥远的鱼类东方鲀具有的编码和非编码的保守片断。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后,使得对DNA的研究不断深入,但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子,还是目前最令人关注的基因工程、染色体绘制等,都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂,目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶,但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短(约为4~8个碱基对),而且一般要求回文结构,这就给飞速发展的基因工程技术带来一定的限制。

T includes three correlative genes: IL-1A, IL-1B and IL-1RN. They code IL-1 alpha, IL-1 beta, and IL-1ra partitively.

L-1 基因簇包含IL-1A、IL-1B 和IL-1RN 三个相关基因,位于人类染色体2q13-q21,分别编码细胞因子IL-1α、IL-1β和IL-1ra。

The field of this course mainly studies the chromosomal disorder, single gene disorder,polygenetic disorders,genetic diagnosis and counseling.

本课程的五个重要内容包括染色体病、单基因遗传病、多基因遗传病、遗传学诊断与遗传咨询。

Conelusion:Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CN Ⅱ case in the family and recessively inherited.

Tyr486Asp突变是本例CN—Ⅱ家系的致病性基因,该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件,其遗传规律符合常染色体隐性遗传。

Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CNⅡ case in the family and recessively inherited. The mutation has no influence on the response elements of phenibarbital in the promoter region.

Tyr486Asp突变是本例CN-Ⅱ家系的致病性基因,该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件,其遗传规律符合常染色体隐性遗传。

In this revie w, chromosome, gene production and phe...

本文综述了8个已应用于商业化育种的甜玉米胚乳突变基因的染色体位点、编码的产物及其表现型,介绍这8个甜玉米胚乳突变基因的分类、遗传效应及其互作效应,并提出其在我国甜玉米育种中的应用策略,旨在为甜玉米的深入研究和开发利用提供参考。

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