染色体基因

The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明，Am6-4中的抗白粉病和抗条锈病基因均为单显性基因；以124对D基因组SSR引物进行标记分析，引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带，标记与抗白粉病基因间的遗传距离为20.42cM，并将抗白粉病基因定位于6D染色体；引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带，标记与抗条锈病基因间的遗传距离为8.0cM，并将抗条锈病基因定位于1D染色体。

Androgen receptor gene located on the X chromosome was first found as a susceptibility gene of AGA. Further more, the sex-determining gene located on the Y chromosome and the hairless gene on the autosome gene were also associated with AGA.

第一个发现的雄激素性秃发易感基因-雄激素受体基因，位于X染色体上；另外，位于Y染色体上的性别决定基因、常染色体上的脱发基因等也与雄激素性秃发发病有关。

Literature Research The etiological factors of POF are not still clear. The changes of sites and numbers in X chromatosome, X; autosome translocations, a forkhead transcription factor gene (FOXL2) mutations, FRAXA premutations and inhibits gene mutations are found in POF patients, so genetic disorders have been thought to be involved. Irradiation, chemotherapy or pelvic operations can damage ovaries and cause ovarian atrophy and failure, so it is thought one factor of POF.

一、文献研究 POF的确切病因目前尚不清楚，从POF患者体内检测到X染色体上位点和数目的变化，X染色体与常染色体间的平衡易位，脆性X基因前突变，FOXL2基因突变，抑制素基因突变等，故认为POF发生与遗传因素有关；由于放射、化疗或盆腔手术等可损伤卵巢，导致卵巢萎缩，出现卵巢衰竭，故也是导致POF的因素之一。

The results of our study have demonstrated that,(1) quadrivanlents in diakinesis Ⅰ are ideal materials for micromanipulation;(2) compared with the instrument used to microisolate chromosomes with a dissecting needle perched on a dissecting microscope outfitting with a micromanipulator or laser beam, the equipment most common used in our study is relatively inexpensive and the techniques could be established as a standard procedure in the ordinary laboratory;(3) the gene mapping methods developed in the study is an efficient and fast approach that could advance gene mapping of fish and other species.

本研究结果表明，（1）减数分裂Ⅰ终变期染色体标本是进行染色体显微操作研究的理想材料；（2）与显微操作仪和微激光分离相比较，本研究所探索的显微分离染色体方法不需要昂贵仪器，在常规实验室即可操作，具有广泛的普及应用意义；（3）把染色体显微操作应用于基因定位这种构思是完全可行的。这种基因定位方法简明而快捷，从而为鱼类及其它物种染色体定位研究开辟了一个新的研究方式和途径。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

Hypotrichids ciliate macronucleus chromosome was named "genesize chromosome", which carrying the gene coding sequence flankedby short non-translated regions and bounded by telomeres (C_4)_4(A_4)_3.Macronucleus chromosome is furthest simple chromosome ineukaryotic cell.

下毛类纤毛虫的大核染色体被称为"基因大小染色体(gene-size chromosome)"，其含有一个基因编码序列，5′和3′端各有一段基因表达调控序列以及端粒序列(C_4)_4(A_4)_3，是真核生物中结构最简约染色体。

It was named DDx24.The sequence of 0876g05 is 100% homologous to one segment sequence of humanchromosome X.Both genes have been registered in GenBank.

可以确定此基因为人类新基因，并经国际基因命名委员会命名为人DDx24基因；基因0876g05核苷酸序列与人类X染色体上一段序列100%同源，未发现同源性大于80%的已知人类基因，同样确定此基因为人类新基因，将该基因定位于人类X染色体Xq2

Photosynthetic traits of Ningai 1 and its 4B-monosomic, nullisomic line were investigated in this paper(Rht3 gene was located in 4B-chromosome). 4B-chromosome has the positive effects on photosynthetic rate, chlorophyll content, RuBP carboxy lase content and activity, leaf conductance, chlorophyll relatve steady phase and photosynthetic avtive duartion and these effects are accumlative. So 4B-chromosome has the promoting influence on photosynthesis.

本文系统地研究了带有Rht3基因的4B染色体二体(宁矮1号)、单体(宁矮1号M4B)、缺体(宁矮1号N4B)材料的光合特性，发现带有Rht3基因的4B染色体对光合速率、叶绿素含量、RuBP羧化酶含量及活性、叶片导度均有正效应，并有累加作用，而且对叶绿素含量缓降期和光合速率高值持续期也有正效应，因此带有Rht3基因的4B染色体具有促进光合作用的效应。

P210bcr/abl plays a central role in the pathophysiology of CML. The purpose of this study was to construct a cell line model that bcr/abl expression can be regulated by Tetoff inducingexpressionsystem. The fulllength b3a2 bcr/abl cDNA was subcloned into the pTRE2hyg expression vector to construct the pT2P210 plasmid.

CML是目前致病分子信号基础研究最为清楚的肿瘤之一，1960年Nowell和Hungerford在CML患者的白血病细胞中发现Ph染色体［1］，1973年Rowley等［2］进一步应用染色体分带技术证明，Ph染色体是由9q34的abl基因和22q11 的 bcr 基因相互易位形成的 bcr/abl 融合基因。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。