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染色体基因

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So far transgenic knockout models of AQP1, AQP3, AQP4 and AQP5, and a knock-in model introducing a point mutation (T126M) that causes autosomal recessive nephrogenic diabetes insipidus in human have been successfully established. Significant progresses have been made in characterizing the physiological functions of these AQPs by systematic mouse phenotype studies.

目前AQP1、3、4、5基因敲除和AQP2基因点突变的基因敲入小鼠模型(模拟人类常染色体隐性遗传尿崩症)已成功建立并广泛用于表型研究,在AQP水通道蛋白生理功能方面获得许多重要进展。

Duke University researchers used two genetic research shows that Nosema is clear from the sexual evolution of the fungi, and in particular with the bacteria closely related to the joint, they found that Nosema of more than 2000 genes, 33 Gene junction with the bacteria, these chromosome markers show that Nosema bonding with the bacteria is likely to have a common ancestor, and it with other known contact fungi grew apart.

杜克大学的研究人员使用两个基因研究证明,微孢子虫明显地从有性的真菌中进化,并且尤其同接合菌密切相关,他们发现,微孢子虫的2000多个基因中,有33个基因同接合菌一样,这些染色体标记显示,微孢子虫同接合菌很可能拥有共同的祖先,并且,它同其他已知的真菌联系更为疏远。

Restriction map of H. polymorpha CBS 1976 chromosomal locus containing △9-fatty acid desaturase gene was constructed based upon the data of Southern analysis, and a 3. 4kb BamHⅠ-XhoⅠ genomic fragment was isolated. Sequence analysis showed that it contains an ORF of 1353bp with high homology with cloned yeast △9-fatty acid desaturase genes.

构建了H.polymorpha CBS 1976染色体△9-脂肪酸去饱和酶基因座位的限制性酶切图谱,进而分离了3.4kb BamHⅠ-XhoⅠ基因片段并进行全序列分析,结果表明这个片段含1个与已克隆的酵母△9-脂肪酸去饱和酶基因高度同源的、由1353bp组成的ORF。

Methods: Two tag SNPs (rs2439322 and rs16879809) of NRG-1 in 254 schizophrenia trios were genotyped by using PCR-RFLP, and LD, TDT and HHRR were analyzed by using EMLD, UNPHASED and TRANSMIT softwares.

目的 :探索位于 8 号染色体短臂( 8p )的神经胶质细胞生子因子 1 ( NRG-1 )基因与精神分裂症易感性之间的关系;探索同样位于 8p 的 NRG-1 基因与 ASAH1 基因之间的相互作用。

Methods PCR amplification of drug resistance gene abeM was carried out using chromosomal DNA of Ab 35 and Ab 36 clinical isolates as template. PCR product of abeM gene was sequenced. The nucleotide sequences and deduced amino acid sequences were analyzed by BLAST of NCBI. After cells of Escherichia coli KAM32 transformed with a plasmid carrying the abeM gene PCR product, MICs of various drugs were determined by serial twofold dilution method.

以临床分离的Ab 35、Ab 36染色体DNA为模板扩增abeM基因,对abeM基因扩增产物进行DNA测序及同源性分析;将abeM基因扩增产物与载体连接后转化入大肠埃希菌KAM32感受态细胞中,对转化子进行药敏分析。

The PCR amplification results of BADH premier showed that the progeny materials that aroma rooted in Xinxiang B could produce obvious DNA amplification bands, whose aroma genes and the aroma genes in KDLMIOS and Basmati fragrant rice varieties all Jay on chromosome 8 and belonged to alleles.

BADH引物PCR扩增结果表明,香味来源于新香B的后代材料能产生明显的DNA扩增带,其香味基因与KDLM105以及Basmati香稻品种的香味基因均位于第8染色体上,属于等位基因。

In order to develop a new path for gene mapping of fish, the digoxigenin and tritium labeled in situ hybridization techniques combined with multiple banding analysis on the pachytene bivalents of M. albus were studied using the wheat ribosomal RNA genes, pig growth hormone gene and MaSoxl (M. albus SRY boxl) DNA segment as the probes. Moreover, the techniques of random primed in situ DNA synthesis were also developed on the bivalents of M.

以两种异源基因和Ma Soxl为探针,采用地高辛或氟标记的原位杂交技术,结合点渍法和DNA印渍法分析,率先在黄鳝减数分裂二价体上开展基因定位研究,并与多重带带型分析相结合,以期为构建黄鳝染色体框架图奠定初步基础,为迅速改变鱼类基因定位研究的落后局面而开辟新的途径。

The stage which is most sensitive to high temperature is trachea colouring embryo(ji-3), while the stage sensitive to dry condition is body pigmentation embryo(ji-5), so these two development stages have been called high temperature sensitivity stage and dry condition sensitivity stage respectively. The gene of high temperature and dry condition sensitivity is defined as sex-linked temperature sensitivity gene, which exists in 21.5 location of Z chromosome linking closely to sch gene, and presents concealed heredity.

其中对高温最敏感的时期是气管显现期(己_3),对干燥最敏感的时期是转青期(己_5),这两个时期分别称为高温敏感期和干燥敏感期,高温干燥敏感性基因定义为伴性温敏基因,t与Z染色体上21.5位置的sch基因紧密连锁,呈隐性伴性遗传。

Nematode assays showed that the resistance to root-knot nematodes was significantly improved in some transgenic lines compared to untransformed susceptible control, and the resistance was inheritable in their selfed progenies.

目前国际上已从番茄中发现了8个抗根结线虫基因,其中3个抗性基因Mi-1、Mi-3和Mi-5在番茄染色体上的定位已经清楚,这篇文章没有明确是Mi基因家族的哪一种?

According to its position on chromosome and resistance spectrum analysis, we believe that Yrhe is a new stripe rust resistance gene.

根据该基因在染色体上的位置与抗病谱分析,认为该基因可能是1个新的抗条锈基因,暂定名为YrHe。

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