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染色体基因

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The dominant male sterility was expressed normally in the new Taigu genic sterile wheat carrying the intergenomically translocated Ms2, and the female fertility mechanism in its male sterile plants was normal as well. Observation of the chromosome configuration at meiosis of pollen mother cells of the young ears of the sterile plants showed that they were euploid plants (2n=42). No configurations different from those of the Taigu geneic sterility gene located at the original locus were noticed of the Ms2 intergenomically translocated back into the common wheat.

所获携带易组Ms2基因的新型太谷核不育小麦其显性雄性不育特性表达正常,且雄性不育株的雌性可育机制正常,对不育株幼穗花粉母细胞减数分裂期染色体构型的观察可见其为整倍体(2n=42),尚未发现回归普通小麦的易组太谷核不育基因与原位点的太谷核不育基因有不同的表型。

Homozygosity for a haplotype that was identical by descent between two of the affected individuals identified a locus for the disease gene within a 17.4 Mb interval on chromosome 15, a region containing 296 genes.

对两个受累个体完全相同的遗传单倍型纯合性分析发现,疾病基因的基因座在第15号染色体17.4 Mb的区间内,该区域包含296个基因。

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中,与感受态的脑膜炎双球菌混合培养,重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取,整合到染色体上,目的基因被带有卡那霉素基因插入的同源性重组片段代替,导致失活,在选择性培养基上筛选出基因被敲除的突变株。

In this study, we successfully localized the gene responsible fornon-syndromic postaxial polydactyly typeA/B to a 2.5Mb region and analyzedgenes and ESTs in the critical region.

本研究成功地将一个新的遗传性轴后多指家系相关基因确定在19号染色体2.5Mb的范围内,并对关键区域内的基因和EST进行了初步分析,为进一步分析该致病基因的突变奠定了基础。

The genic polymorphism is that there are two or more genotypes in the same locus of chromosome in the group of random mating. It is an important factor to determine the susceptivity of desease, the diversity of clinical symptom and the reaction of medication.

所谓基因多态性是指在一随机婚配的群体中,染色体同一基因座位点上有两种或两种以上的基因型,它是决定人体对疾病易感性、临床表现多样性及药物治疗反应差异性的重要因素。

Uptilt now it has been established that this kind of genes like hsMAD2 may control the primary carcinogenesis, Since invalidity of the checkpoint controlled by MAD, ie.

目前认为以该基因为代表的这类基因可能控制着最初的癌变过程,因为MAD基因控制着的关卡的失效会导致染色体畸变和基因组的不稳定。

Uptilt now it has been established that this kind of genes like hsMAD2 may control the primary carcinogenesis, Since invalidity of the checkpoint controlled by MAD, ie. Spindle assemble checkpoint will result in deformation of chromosome and instability of genome.

目前认为以该基因为代表的这类基因可能控制着最初的癌变过程,因为MAD基因控制着的关卡的失效会导致染色体畸变和基因组的不稳定。

The analysis of the mutants of amrA and amkA showed that the production of rifamycin SV had decreased about 10%-20%.

用双交换法在地中海拟无枝菌酸菌U32染色体上插入阿泊拉霉素抗性基因,成功地构建了了amrA,amkA和amrB基因的突变株,其中amrA,amkA基因突变株的菌丝形态与野生型U32之间有较明显的差别,在菌丝的末端形成卷曲或弯曲。

Comparabilityof some segments reached about 86% to 90% including Arabidopsis thaliana CIPK7、Arabidopsis thaliana At3g23000 gene、Arabidopsis thaliana SNF1 related protein kinase(At3g23000; MXC7.3) mRNA、Cucumis sativus RBL1 mRNA, complete cds、Ranunculusmacranthus ribosomal protein L14 (rp114) gene, partial cds、Daucus carota chromasomecomplete genome and so on. There are also some other segments had a low identity andconsidered as unknown genes.

对部分差异条带进行了克隆测序,登陆GENEBANK对差异cDNA片断的序列进行核苷酸及氨基酸的同源性对比,其中一些片断与拟南芥CIPK7、拟南芥At3g23000基因、拟南芥SNF1相关蛋白激酶mRNA、栽培黄瓜RBL1mRNA全序列、毛茛核糖体蛋白L14基因、胡萝卜染色体基因组等均有86%-90%左右的同源性,还有部分片断的同源性很低,可能为未知基因序列。

The Sry gene sequence of Muntiacus crinifrons was acquired and the conclution was that there are 83% homology between the human and Muntiacus crinifrons. It was testified that in all mammal Sry gene is consertive. On the other side the Sry gene was located to the Y2 chromosome of the Muntiacus crinifrons.

最后提取雄性黑麂的基因组DNA,并用同一对引物对其进行扩增,亦得到Sry基因的片段,对此扩增片段进行克隆,测序,结果表明其与Y2染色体得到的Sry基因片段完全一样,与人SRY基因的同源性均为83%。

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