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The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅,属鲈形目、刺鳅科(Perciformes,Mastacembelidae),是广泛分布于中国南方的小型淡水鱼类。1998年,我室通过核型分析,首次发现该物种具有明显分化的异形性染色体,其性别决定类型为XX/XY型。1999年,本室通过染色体显带、精母细胞联会复合体观察,再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体,两者不仅已经出现结构异染色质分布上的不同,同时在外部形态上也存在明显差异,识别特征鲜明,在光镜观察下无需显带即能清晰辨认性染色体

Chromosome pairing behavior in meiosis of the hybrid F1 was carried out. Results showed that in an average , 1.25 rod bivalents were observed in one PMC, meiotic configuration was 2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25) and most of PMCs possessed 1~5 bivalens, indicating that the relatively high homeology was detected between the D genome of Ae.tauschii and the V genome of D.villosum. The morphological differences between F1 hybrids and their parents were significant. F1 plants were highly self-sterile, but partially self-fertile after treated by chromosome doubling technique.

对杂种F1花粉母细胞减数分裂中期Ⅰ染色体配对行为进行观察发现,"节节麦×簇毛麦"杂种F1平均每PMC有1.25个棒状二价体,染色体的平均构型为2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25),大部分被观察的细胞出现1~5个二价体,表明节节麦D染色体与簇毛麦V染色体间具有相对较高的部分同源配对, D和V染色体之间存在一定的部分同源性。F1植株高度自交不育,经染色体加倍处理后能够自交结实。

63 All are the telocentric chromosomes. The sex chromosomes ZZ in the male are subtelocentri chromosome, which are the largest chromosome pair in the whole genome.

雄性性染色体ZZ是整个染色体组中最长的一对近端着丝粒染色体;雌性的W染色体也为近端着丝粒染色体,其长度仅次于第3号常染色体

NO.1 chromosome was submetacentric,NO.2 and Z chromosome were metacentric,and the others were telocentric,which was slightly different from the results of others. Additionally,analysis of G-banding patterns for macrochromosomes indicated that they were divided into 27 zones and 134 bands.

染色体形态分别为:NO.1染色体为sm型,NO.2、Z染色体为m型,其余染色体均为t型,这与前人研究结果存在一定差异。G带分析结果显示,鹌鹑的前9对大染色体及Z、W染色体G带可分为27个区,134带。

Three kinds of embryos' chromosome, including goat fertilization embryo, goat-goat cloned embryo and goat-rabbit cloned embryo were analyzed, The results showed that all of them have 60 chromosomes with same character, all were telocentric chromosome, besides the X-chromosome is the second larger telocentric chromosome.

经对超排获得的山羊受精胚、山羊同种克隆胚和羊-兔异种克隆胚的囊胚期胚胎染色体分析表明,三种来源的胚胎其染色体数目均为60条,且所有常染色体均为端部着丝点染色体,X染色体为第二大的端部着丝点染色体,说明山羊同种克隆胚和山羊-兔异种克隆胚均为山羊的核型,是供体细胞核在不同胞质中的重新编程的结果。4。

Effect of Different Sowing Date and Densities to the Yield of Avena nuda L .Variety Huazao No.2;2. The paper analyses the chromosome karyotype of Avena nuda L .

对莜麦染色体的核型进行了分析,结果表明其染色体数为42,为六倍体,包括11对中部着丝粒染色体,9对近中部着丝粒染色体和1对近端着丝粒染色体,其中有3对染色体带有随体。

All 29 pairs of autosomes were acrocentric chromosomes;X chromosome was the second largest acrocentric chromosome and Y was the smallest and the only one metacentric chromosome in all chromosomes.

所有常染色体均为端部着丝点染色体;X染色体为第二大的端部着丝点染色体,Y染色体为最小的且是唯一的中部着丝点染色体

Thirty six homologous Pairs of chromosomes were autosomes, in which chromosomes, Pairs of 1 to 8 were metacentric.9 to 36 . And X was metacetric, Y chromosome terminal.

其中,1—8对常染色体为中间着丝粒染色体,9-36对常染色体为端部着丝粒染色体,X为中着丝粒染色体,Y为最小的为端部着丝粒染色体

When applied the spiny eel X chromosome library probes to the chromosomes of the two fish, different signals were distributed: in Siniperca chuatsi, besides some little signals on lot of autosomes, a large bright signal region was detected on the 8th chromosomes, which represented a synteny of spiny eel X chromosomes; but in Channa argus, no distinct synteny was found.

当以刺鳅的生物素标记X文库探针对鳜鱼和乌鳢的有丝分裂染色体进行染色体涂绘后,经过核型分析和统计分析表明,在这两种鱼的染色体上的杂交信号分布是不同的:在乌鳢中,除散在信号外,在其第八号染色体近着丝粒部位存在着一段较大的信号结合区域,应是与刺鳅的X染色体同源的保守同线群,而在鳜鱼中,只有散在信号分布于多条染色体上,没有明显的保守同线群存在。

Chromosome number in all counted complete metaphases of Carassius auratus var. Dongtingking is 48. Analysis of chromosome can be divided into four sections according to the position of the centromere.

核型分析结果表明,翘嘴鲌的肾细胞染色体组是由48条染色体组成,染色体组型按着丝粒位置可分为四组,分别是中部着丝粒染色体、亚中部着丝粒染色体、端部着丝粒染色体

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