染色体

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明，茎尖分生组织培养的幼苗及丛生苗遗传稳定，其染色体未发生倍性变异，均为2n=16；愈伤组织及其再生苗遗传稳定性较差，愈伤组织染色体数变异率为43.4%，其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%；愈伤组织分化苗染色体变异率为11.7%，其中单倍体占6.7%，三倍体占1.7%，四倍体占3.3%。

GISH analysis showed Triticale have 2 items chromosomes presented yellow and others chromosomes presented chrysoidine, because yellow chromosomes combined the Thinopyrum intermedium DNA, others chromosomes dye by PI. 4. Analysis of resistance gene: CH-9916 is the hybrid progenies of Octoploid tritielytrigia TAI-7045 X common wheat "Jinmai33".

GISH分析结果表明，小偃麦衍生系CH-9916的42条染色体中有2条外源染色体因结合有探针DNA而呈现出较强的黄色杂交信号，而没有结合上探针DNA的普通小麦染色体经过PI染色呈现出桔红色，初步推断小偃麦衍生系CH-9916为一个"小麦一中间偃麦草"的代换系。

Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats,but the signals were not the same on different chromosomes in intensity.

粗线期染色体荧光原位杂交结果表明，大多数染色体的末端都有端粒串联重复，但信号的强度在不同染色体上是不同的。

Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats,but the signals on different chromosomes were not the same in intensity.

粗线期染色体荧光原位杂交结果表明，大多数染色体的末端都有端粒串联重复，但信号的强度在不同染色体上是不同的。

FISH with the tomato 45S rDNA clone revealed very strong signal in the satellite on the short arm of chromosome 2 as well as weak signals in five CPD banded regions at pachytene or four pairs of CPD banded regions at metaphase.

用番茄的一个45S rDNA 克隆进行的荧光原位杂交，不仅在位于2号染色体短臂的随体上显示了强的杂交信号，而且在粗线期染色体的5个CPD带区或有丝分裂中期染色体的4对CPD带区显示了弱的杂交信号。

Acquisition of high-quality chromosome suspension: The chromosome suspension released from homogenization suited for flow karyotypic analysis and sorting after fixed in 2% paraformaldehyde for 20 minutes and purified through 10% and 30% sucrose concentration gradient.

高质量染色体悬液制备结果：同步化的根尖细胞经2%多聚甲醛固定20 min，机械匀浆释放染色体并结合10%及30%蔗糖浓度梯度纯化释放的染色体悬液。

The applications of chromosome numbers and basic numbers in pteridophyte systematics are reviewed in detail. The significance of karyotype analysis, polyploidy, pogamy, and reticulate evolution in pteridophytic classification, evolution, and phytogeography is also reviewed.

文中论述了蕨类植物染色体数目和染色体基数在系统学上的应用以及蕨类植物染色体组型分析、多倍化、无融合生殖、多倍体复合体及网状进化的研究概况。

Puna Chicory, leymus multicaulis and Psathyrostachysjuncea.For the materials used in this article were some old seeds which had been stored for 3-7 years, we found a lot of abnormal mitotic and meiotic cells, such as chromosome bridge. chromosome laggard, micronucleus, multipolar division and so on. Moreover, we also found the wheat"s multi-anther, double germinate-hole"s microspore and collected the photos of every period of meiosis. mitosis.

因为用于细胞学检测的小麦材料是搁置多年的陈旧种子，所以在检测染色体数目时，发现大量异常分裂现象，包括染色体桥、染色体落后、微核、多极分裂等；实验过程中还发现小麦多花药现象和花粉粒具双萌发孔的现象；同时在显微观察同时还注意搜集了细胞有丝分裂、减数分裂各个时期的分裂相图片。

Purpuraristatus, the growth, fertility, chromosome configuration, EST isozyme etc. were analyzed in this paper. The results showed that the growth potentiality of (E. sibiricus×E. purpuraristatus) F2 and (E. purpuraristatus×E. sibiricus) F1 were much stronger than their parents, the plant height of the former was 143.2 cm. The whole plant was reseda, the latter was 129.7 cm. The spike nodding of two hybrids were in the middle of their parents, the anther was yellow; the pollen fertility was 0.02%~0.03%, seed set was 0; the average chromosome configuration of former pollen mother cell at PMC M Ⅰ was 6.90Ⅰ+14. 02Ⅱ, the latter was 7.82Ⅰ+13.59 Ⅱ, and lagging chromosome and bridge fragment were observed at meiosis anaphase Ⅰ the EST of the two hybrids F1 and their parents at tillering stage was some certain different in locus, number and intensity.

结果表明，正交F1和反交F1植株的生长势均很强，正交F1株高143.2 cm、全株浅绿色，反交F1株高129.7 cm、全株灰绿色；正、反交F1的穗型均呈双亲中间型，花药呈黄色，花粉可育率0.02%~0.03%，结实率为0，说明杂种高度不育；正交F1的花粉母细胞减数分裂中期Ⅰ平均染色体构型为6.90Ⅰ+14.02Ⅱ，反交F1为7.82Ⅰ+13.59Ⅱ，减数分裂后期丁有落后染色体和染色体桥等不规则现象；亲本及其正、反交杂种F1分某期幼叶的EST同工酶酶带的位点、数目和强弱均存在一定差异，可作为亲本及杂种在蛋白质水平识别的重要依据。

This is the first fish sex chromosome specific repetitive DNA sequence which was found in China so far.

将上述片段的探针对刺鳅染色体进行FISH分析后，结果显示，其中RMX6对于X染色体是特异的，其杂交信号仅限于X染色体上异染色质区。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。