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METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠，链脲佐菌素（streptozotocin，STZ）腹腔注射，制成糖尿病模型，于模型建立2wk后，大鼠眼玻璃体内注射BDNF溶液，于模型建立4wk后，处死大鼠，取出眼球，取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测，观察视网膜中TH水平的变化，从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果：电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀，主要分布于神经元的胞浆中或突起内，神经元分化的初期，溶酶体集中分布于胞质内，随突起的长出，可见在突起内出现。

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The authors proposed that heterochromatin change in genome might play an important role in balancing the germplasms between maize and its distant relatve after cross and parthenocarpy.

这种变异不仅存在于表型不稳定的早代孤雌系中，而且还存在于表型稳定的高代系中，造成这种变异的原因可能是因为远缘种质的引入影响到遗传平衡进而引起染色体发生结构重排并引起染色质的变化，这种变化与玉米染色体组中非同源染色体间存在的同源片段和因此造成的减数分裂过程中非同源染色体配对有着直接的关系。

ERK positive cell mainly distributed at nucleus of solitary tract, ambigous nucleus, and positive fibers and cells mainly distributed at medullary visceral zone, nucleus of solitary tract and ambigus nucleus. These positive cells formed an isolated zone from nucleus of solitary tract via medullary visceral zone to SP5, which could be seen using immunohistochemical stain. Positive cells and fibers also distributed at the projection from pneumogastric nerve to the stem.

免疫组织学染色可见，阳性细胞主要分布于延髓内脏带的孤束核和疑核、阳性纤维伴随阳性细胞分布于延髓内脏带的孤束核区和疑核、呈沿孤束核经延髓内脏带至三叉神经脊束核的弧行带；在迷走神经向脑干投射部位也可见阳性胞体和纤维的分布。

The rats were killed in 0,24,48 and 72 hours after hypoxic-ischemia to detect the level of SOD and NDA in rat brain and neuronal apoptosis in the hippocampal CAI regin was detected by HE and TUNEL staining.

于手术后即刻，②、③于处置后0、24、48、72h断头取脑，分别检测SOD/MDA的水平，并经HE和Tunel染色，光镜下检测海马CAI脑细胞凋亡数。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

Result: Immunohistochemic results indicate that after HBO treatment, the expression of CytC and Bax in the neuronal cytoplasm decline significantly ( P.05) except in the time of 72 hours after TBI; the Bcl-2 expression is up-regulated significantly(p.05) after TBI. The images of electromicroscope indicate that before 24hr after TBI, mitochondria of control groups become more swoln even fracted and the matrixes become vaguer than those of being treated with HBO.

郑州大学2004年硕士研究生毕要论文高压氧对大民实脸性脑顶伤后神经细胞内cytochr她eC，Bol一2以及Bax表达的影响结果：(1)对于致伤后的脑组织切片染色发现，相同时间段内(3，6，12，24小时)实验组神经细胞内CytC阳性表达明显低于对照组(P.05)，而72小时以后无明显差异；Bel一2阳性表达于3，6，12，24，72小时时明显高于对照组(P.05)，Bax阳性表达于3，6，12，24小时时明显低于对照组，72小时时无明显差异。

The choosing of the material and staining of Golgi body and making section;2. To determine the subcellular localization of the encoded protein of a transcriptionally up-regulated gene pdd87in the pten knockout mice,fusion protein expression vector pEGFP-C 1 -pdd87was constructed and transfected into NIH3T3cells and cells were stained with BODIPY TR C 5 -Ceramide to display Golgi body .

为了明确pten敲除小鼠中转录上调基因pdd87编码蛋白在细胞中的定位，构建PDD87与绿色荧光蛋白的融合蛋白表达载体pEGFP-C1-pdd87，利用Lipofectamine2000转染该载体于体外培养的NIH3T3细胞中，采用高尔基体特异探针BODIPYTRC5-Ceramide染色显示高尔基体，激光共聚焦显微镜下观察到PDD87-GFP融合蛋白定位于高尔基体，提示pdd87基因编码的蛋白定位于高尔基体。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。