染色于

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

More thicker epiphyseal plate were found in the left femurs than the right femurs of the group fixed with plates at 3 and 6 month after operation. In the group fixed with plates, Safranine O staining showed epiphyseal plates ot the left femurs had more fuscous staining than the right femurs at 3 and 6 month after operation. Electron microscopy also found that the cells of the epiphyseal plates of left femurs were more eugenic than the right femurs in the group fixed with plates at 3 and 6 month after operation.

光镜学实验结果示滑动钢板固定的左侧股骨骨骺板总厚度及肥大细胞计数均明显大于普通组；组织化学观察示滑动钢板固定的左侧股骨骺板于术后3个月及6个月Safranine O组织化学染色明显深于普通组；电镜观察示滑动钢板固定的左侧股骨骨骺于术后3个月及6个月软骨增殖区软骨细胞分裂及生长较普通钢板固定的右侧活跃。

That to explore the general biologic mechanism of acupuncture through observation of ERK1/2 and P38 is a new try of studying the mechanism of acupuncture in the fascial point of view.

综上所述，本研究分别采用了HE染色、免疫组化、免疫印迹等技术手段，从针刺作用于机体的始动环节——针体对浅筋膜的牵拉和对肌肉的刺激着手，区别于以往的&穴位刺激——靶器官反应&的对于针灸研究的传统模式，且从观察ERK1/2和P38两种信号蛋白变化的角度，来探讨针刺作用于机体在生物学层面的普遍的机制，这是从筋膜角度研究针刺机理的一个新的尝试。

1Cubic millimeter tissue sampling was fixed with the mix of 1% glutaraldehyde and 4% paraform in 4℃.

利用兔后腿软组织损伤、海水浸泡模型，分别于损伤后浸泡于海水中2小时，浸泡后分别进行各种低渗盐水冲洗，分别于干预处理后1h、3h、6h、8h、12h，在损伤周围0.5cm范围内的肌肉组织中取材，0.5cm3损伤组织用4%福尔马林固定，HE染色，光镜下观察损伤组织的细胞浸润情况，用免疫组化法检测受伤肌肉组织凋亡相关基因Caspase3表达情况；1mm3损伤组织用1%戊二醛一%多聚甲醛混合固定液(pH7.4 ) 4℃固定，电镜观察其细胞凋亡及细胞膜、线粒体等结构的变化；生化检测Na+，K+-ATPase活性，了解其组间的差别。

Methods BTXA labeled with125Iwas obtained. The radioactivitymeasurementwas taken tomeasure the solubility ofBTXA in gel and BTXA solution. The rightgastrocnemiusmuscles of12 SD ratswere injectedwithBTXA geland the leftgastrocnemiusmuscles injectedwith normal salinewere used as controls. HE staining and 1% gold chloride stainingwere used to study the tissue changes 6 months and 12 months afterBTXA gel injection and transmission electronmicroscopy was used to observe the ultrastructural changes.

将BTXA行示踪标记配制125I-BTXA水凝胶与125I-BTXA水溶液，进行样品放射性强度测定。12只SD大鼠用随机数字表法分为A、B两组，每组6只，大鼠右侧腓肠肌注射BTXA水凝胶，左侧腓肠肌注射等体积生理盐水作对照，于注射后6个月、12个月切取腓肠肌标本，常规病理HE染色、1%氯化金染色，透射电子显微镜观察组织结构变化。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论：成骨细胞呈梭形，多突起；细胞核呈卵圆形，偏于一侧；胞体大，胞浆丰富；碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒，某些部位染成黑色，定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞；细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

The rabbit osteoarthritis model were duplicated by Hulth's technique. The pathologyical changes of each group was observed with naked eyes before taking the thighbone department carilage of pulley block and the platform place of shine bone carilage, then under the light microcope and the transmission electron microcope respectively, after treating with Semer Strychni (high、middle and low dose) for two months.

按Hulth法造兔膝骨关节炎模型，用马钱子高、中、低剂量进行灌胃治疗，连续2月后取材，进行肉眼大体观察后，取股骨滑车部软骨及胫骨平台处软骨，分别于HE染色后光镜下观察和铀-铅双重染色透射电镜下观察各组的病理变化。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

The main products are: T / C printing / dyeing cloth, chenille fabric dyeing, dyed, coated cloth, which are widely applied to curtains, bedspread, cushion, sofa, etc. produced.

主要产品有：T/C印花布/染色布、雪尼尔染色布、色织布、涂层布等，产品广泛适用于窗帘、床罩、靠垫、沙发等的制作。

Method］Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法］rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养，应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子，并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物，直接种植到兔膝关节实验性关节软骨缺损处，并与对照组相比较，观察软骨修复效果。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。