染色于

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Moreover, all the signals on X and Y only located in the heterochromatic regions, showing the same distribution with those of C-band.

所有X染色体上的信号是一致的，而X、Y染色体上的信号分布则彼此不同。X、Y染色体上的杂交信号区域均集中于异染色质区，信号分布模式同X、Y上异染色质分布模式完全吻合。

In the present study, the expression of bFGF and TGF β1 in normal and hyperplasic prostate were determined with immunohistochemistry and in situ hybridization.

本研究采用免疫组化及原位杂交方法检测bFGF和TGF-β1在正常前列腺及BPH中的表达情况，结果显示，正常及增生前列腺组织中上皮及间质细胞中均见bFGF、bFGFmRNA及TGF-β_1、TGF-β_1mRNA阳性染色，间质细胞染色强于上皮细胞。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5cm above the dome of acetabulum, which were fixed with ATMF'S or steel plate respectively. The animals were sacrificed and specimens were procured at 2, 4, 6, 8 and 12 weeks after operation. Samples from the fracture gaps were investigated by histology of HE and Masson staining, image pattern analysis of new bone formation.

方法］ 选用10只成年杂种家犬，双侧髋臼臼顶上方1.5cm处横形截骨，分别采用ATMFS（骨盆髋臼三维记忆内固定系统）前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

Method］Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum, which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

方法］选用10只成年杂种家犬，双侧髋臼臼顶上方1.5 cm处横形截骨，分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

Method］Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum, which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.

］选用10只成年杂种家犬，双侧髋臼臼顶上方1.5 cm处横形截骨，分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

［Objective］To explore the effect of continuous physiological osteogenic stress on the bone healing of canine iliopectineal crest.［Method］Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum,which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

探讨持续顺应生理力线的压应力对犬骨盆弓状线部骨折愈合的影响［方法］选用10只成年杂家犬，双侧髋臼臼顶上方1.5 cm处横形截骨，分别采用ATMFS(骨盆髋臼三维记忆内固定系统)柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

Results Four weeks after surgery, animals in DO group showed an increased voiding frequency, higher voiding pressure and irregular nonvoiding contractions (n=12, P.05). We found increasing mutual connection and multilayer construction in suburothelium and intermuscular layers of DO but not DS and control bladders.

对照组、DS组和DO组豚鼠膀胱均观察到ckit染色阳性且呈典型长梭形有突起的ICC，主要分布于平滑肌肌束间和黏膜下层；在DO组豚鼠的膀胱中ckit染色阳性的ICC分支突起增加且互相连接呈网络状，而在对照组和DS组中ICC呈散在分布，细胞之间联系较松散。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。