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BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Silk is dyed with nine reactive dyes containing various reactive systems and having different reactive groups. After the dyeings being thoroughly extracted to remove all noncovalently bounded dyes,they are treated with buffer solutions in pH range from 1.89-10.65 at 70℃.

丝用九个含有不同活性基团和活性基个数的活性染料染色，着色丝上非共价结合的染料被萃取净后，用pH值从1.89至10.65五个不同缓冲溶液于70℃进行水解，吸附在丝上的水解染料再次被萃取净。

The exhaustion of 3-5a-6 on PLA fabric reaches 98.6%, its fastnesses to washing, sublimation and rubbing reach 4-5 grade, 4 grade and 3 grade respectively.

而磺酰叔胺型染料更适合于聚乳酸纤维的染色，3-5a-6在聚乳酸织物上的竭染率达到98.6％，耐水洗牢度为4～5级，耐升华牢度在4级以上，耐摩擦牢度在3级以上。

It seemed that the staining properties of grass fish mast cells were similar to mammalian mucosal mast cells in some aspects.

草鱼肥大细胞的染色性质在某些方面似乎类似于哺乳动物的粘膜肥大细胞。

The results indicated that four SOD bands were identified in five tissues, and the band with the biggest mobility ratio were found weakly in gill, digestive gland and adductor muscle but not in foot and mantle. Two ATPase bands were found in four tissues except digestive gland. One MDH band was found in five tissues, but the MDH gene expressed strongly in foot and adductor muscle. Two ME bands were examined in foot, adductor muscle and mantle, but the band with smaller mobility ratio was found in gill and digestive gland.

结果表明，SOD在紫贻贝的5种组织中均有表达，共有4条酶带，但迁移率最大的酶带在足和外套膜中未出现，而在另外3种组织中呈弱表达；ATPase在除消化腺外的组织中都表现为2条带；MDH在5种组织中都表达出1条酶带，其中足和闭壳肌的MDH染色最深；ME存在1~2条酶带，其中迁移率大的条带在5种组织中都有表达，而迁移率小的条带只存在于外套膜、闭壳肌和足中。

Methods Hepatic carcinoma cell lines of high and low potentialities of lymphatic metastasis were injected into the footpads of Balb/c mice. Their metastases to lymph nodes were examined. The tumor tissues of each group were stained with 5′-nucleotidase-ALP to observe the lymphoangiogenesis.

将淋巴道高、低转移潜能的肝癌细胞接种于Balb/c小鼠，观察成瘤及转移情况；对肿瘤组织进行淋巴管染色，观察淋巴管生成情况。

The function of plant genomes depends on chromatin marks such as the methylation of DNA and the post-translational modification of histones.

植物基因组的功能依赖于染色质标记，诸如DNA的甲基化和组蛋白的转录后修饰。

Expression of hbFGF in the RPE cells was determined by in situ hybridization and immunohistochemical met hods.

于荧光显微镜下观察GFP的表达情况，用原位杂交和免疫组织化学染色法检测bFGF在RPE细胞的表达情况。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。