染色于

Among its many biological activities, the major function of GM-CSF is to stimulate hematogenie stem cells prolification from silent stage to S phage, so that the stem cells can differentiate into granulocyte-macrophage cells.

有4个外显子和3个内含子，基因定位于5号染色体长臂5q21-5q32区，大多数器官均能产生GM-CSF，GM-CSF有多种生物活性，作用于多种细胞谱系，其主要作用是刺激造血祖细胞增殖即从静止期进入S期，并分化为粒细胞---单核巨噬细胞。

Exploring the basic pathophysiological mechanisms of aneurysm formation and rupture will have great benefit to the aneurysm therapy. Monocytes, fibroblast and T lymphocytes are the main inflammatory cells in the walls of the unraptured and raptured aneurysms.

对象和方法：对2例未破裂动脉瘤和11例破裂的动脉瘤壁分别进行常规HE染色观察，应用免疫组化的方法，用MIP-1α和MCP-1的单克隆抗体，分别观察MIP-1α和MCP-1在蛋白水平于未破裂动脉瘤和破裂的动脉瘤壁内的表达及位置。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Malignant meningioma or rhabdoid meningioma may have similar histopathology, but the immunohistochemical staining pattern shown is not consistent with any antibody commonly used for meningioma.

恶性脑膜瘤或横纹肌样脑膜瘤也可能出现相似的组织病理学改变，但该例显示的免疫组化染色模式不同于任何一种常用于脑膜瘤诊断的抗体。

Methods:By means of IFA and DFA from 1999 to 2000 serological and epidemiological investigations were conducted.

采用间接免疫荧光抗体法和直接荧光抗体染色法，于1999～2000年在平江县选点调查。

The varying of expression of Cfos in different phases and different brain tissue including caudate nucleus, putamen, globus pallidus, hippocampus, nuclei ventrolaterales thalami, parvocellular reticular nucleus, and dorso lateral magnocellular nucleus were examined by immunohistochemical technique.

采用Cfos免疫组化染色，在显微镜下观察Cfos于不同时相在脑膜、脑皮质区及相关核团如尾壳核、苍白球、海马、背侧丘脑腹后外侧核、小细胞网状核、背侧旁巨细胞核的阳性细胞数。

At 1st、2nd、3rd months after surgery the rats were killed respectively and the implanted tibiae were got , the tissue samples were fixed with 10% Formaldehyde soaked and 8% formic acid was used to dissolve the calcium for 10 days.

于种植术后1个月、2个月、3个月时，取出带种植体的胫骨，取下的标本立即放入10%甲醛缓冲溶液固定、经过8%甲酸溶液脱钙、梯度酒精脱水后，制作HE染色的组织学切片，做光镜及骨组织的形态计量学观察。

4The Masson trichrome stain can be used for an important method for the observation of the fibrostic cells, The changes of the mast cell could be considered as an assist marker for the observation of the liver fibrogenesis.In addition the concentration of the hepatic hydroxyproline could be used for diagnosis of the hepatic fibrosis. clinical and experimental study.

Masson三色染色可以作为肝纤维化肝硬化组织细胞观察的一种重要方法：肝纤维化过程中，肥大细胞数量的动态变化，可以作为观察肝纤维化变化的一项辅助指标；肝脏羟脯氨酸的浓度变化可以作为肝纤维化的辅助诊断及应用于相关实验的研究。

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1（TGF-β1） were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只，取肺组织行HE、Masson染色评价肺组织病理变化，消化法测定肺组织羟脯氨酸含量，分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1（TGF-β1）的mRNA和蛋白表达。

P19 differentiation was induced by retinoic acid, and P19 cells cultured on gelatin were used as control. Differentiated cells were observed with microscope. TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation. Survival cells were detected by AlamarBlueTM method.

视黄酸(retinoic acid，RA)诱导P19细胞分化，培养于明胶包被培养板上的P19细胞为对照组，普通光学显微镜观察其神经分化情况，TURNEL法和DNA片段化分析法检测细胞凋亡情况，alamarBlueTM染色法检测细胞存活情况。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。