染色于

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ（ P .05）. There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%（48h after transfection） to 5.63%（72h after transfection）.

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制（ P 。05）；细胞形态发生改变，细胞体积缩小，核固缩，染色质浓缩聚集于核膜；流式细胞术检测显示G 1 峰前出现亚二倍体峰，转染48、72h后凋亡率分别为5.47%和5.63%（ P nbsp； 0.05）。

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示，pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处，而等渗区域的近曲小管细胞几乎无pI〓的存在，据此认为pI〓可能在耐受渗透压的变化上发挥作用。

At 7 and 14 days following transection of masseteric nerve through which Fluorogold was applied to identify the Me5 neurons innervating masseter, brain sections were immunohistochemically processed to detect the three Trk isoforms in FG-labeled Me5 neurons.

通过大鼠咬肌神经给予荧光金，标记支配咬肌的Me5神经元；分别于切断咬肌神经后7和14 d对脑切片进行免疫组织化学染色并观察荧光金标记的Me5神经元表达的三种Trk受体。

Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞，为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性，我们进行下列实验：获取并体外平面培养成体兔骨髓基质干细胞，应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导，诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化，结果证实，诱导后骨髓基质干细胞可分泌Ⅱ型胶原，组织学染色可见类似于软骨细胞，由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化，其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

On the fibre the long olivary SCS connected end-to-end.

S-100蛋白免疫细胞化学染色，长橄榄形SCS突起首尾相连，螺旋式攀缠于纤维上。

In results, immunoreactive neurons of NPY in the New Jianghan chicken's medulla oblongata were located mainly in inferior olivary nucleus, and a few in raphes nucleus and solitary tract nucleus.

免疫组织化学染色结果显示，NPY阳性神经元主要存在于延髓下橄榄核中，孤束核及中缝核也有少量表达。

Result In the control group, oviductal mucosa and glycoprotein showed not significant changes. In the experimental group, the lesions werelimited to theoviductal mucosa. The staining result showed that CT infection decreased the secretion of the neutral glycoproteins.

结果 对照组输卵管粘膜结构和糖蛋白组分无明显变化；实验组输卵管受损部位主要局限于粘膜层，PAS Alcianblue染色显示沙眼衣原体感染可导致中性糖蛋白分泌减少。

Higher degree of staining was observed in epithelial cells than in stroma. Both 5α-reductases were mainly localized in the cytoplasm, while in a number of cells they exhibited a nuclear and perinuclear distribution.

型及Ⅱ型还原酶在前列腺组织中并不存在所谓的特异性的细胞类型分布，两种酶在腺上皮细胞中的表达均强于间质，而且以胞浆的染色为主。

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

POY and FDY oiling agent s on wetting, friction, heat-resisting, attrition-resisting properties, etc.

比较了国产和进口的适用于涤纶高速纺丝的预取向丝油剂及一步法牵伸丝油剂的润湿、摩擦、耐热、耐磨等性能，经实际生产应用证明二者的可纺性、上浆性、染色率基本相同。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。