染色于

The results indicate that heterochromatin and euchromatin can be highly interspersed, even to the point where heterochromatin peaks can exist within the transcribed region of a neighboring active gene.

结果表明，异染色质和常染色质相互渗透，甚至异染色质可以存在于转录区中。

Results Trunk neural crest stem cells were successfully isolated and cultured. The immuocytochemcal result showed that the cells were nestin-and P75-positive. Trunk neural crest stem cells grew through adhering to chitosan fiber after inoculation. Under an electon microscope, the spindle-shaped trunk neural crest stem cells were proliferated and migrated along the fiber. The cells arranged side by side or linked by heads and tails in processes. The ends of S-100 positive cells were flat and expanded, adhering to the fiber as claw-shaped pseudopodium.

结果 采用神经管植块法培养的神经嵴干细胞，免疫细胞化学染色呈现nestin及P75双阳性，将其接种在壳聚糖纤维支架材料上，可见神经嵴干细胞贴附于壳聚糖纤维上生长；S-100免疫细胞化学染色显示，S-100阳性细胞突起的末端呈扁平状膨大；扫描电镜显示，壳聚糖纤维上的躯干神经嵴干细胞为梭形，呈现"端对端"、"肩并肩"排列，伸出爪形伪足贴附于壳聚糖纤维上。

The effect of Alcian blue Safranin O staining was the best, which was applicable to identification of the mast cell of A. Japonica. The improved toluidine blue staining was not applicable.

AB/SO染色法效果最好，适用于日本鳗鲡肥大细胞的鉴定；改良甲苯胺蓝染色法不适合于日本鳗鲡肥大细胞的鉴定。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化，于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色，以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下，于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色阳性，诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

Results:By chlorophyll disclosing solution coloring lymph nodes, we found, by injecting the solution into rectal wall of mouse, it colored lymph nodes quickly(in 30 minutes) and was not easy to precipitate. The dyeing lymph nodes showed emerald green, it contrasted sharply with surrounding tissues and no color fading after injected 24 hours. The average number of lymph nodes observed in each mouse was 6.92, which was more than that of LD1 group significantly(P.01). No adverse reaction or complication were observed in the course of injection and after injection, And the ultrastructure of the lymph nodes colored at different interval was no significant difference to the ones uncolored.

结果：于直肠壁内注射CDS，淋巴结染色迅速，不易沉淀，各级哨兵淋巴结在30 min内被染成翠绿色，与周围组织对比明显；LD2组平均每鼠检出淋巴结6.92个，显著高于LD1组（P.01）；染色淋巴结24 h后仍无褪色，且染色后细胞超微结构无明显改变；术中和术后均未发现不良反应和并发症。

The cation dyeable polyester fiber,which was developed by Do punt corporation in 1958 and commercialized in 1962 known as Dacron,is a main representative of new polyester fibers because of excellent dyeability and low cost.

阳离子可染聚酯纤维首先由美国杜邦公司于1958年研制成功，并于1962年实现商品化，商品名为达克纶，由于其优异的染色性能和低廉的染色成本，使它成为聚酯纤维新品种的主要代表。

The denervated extensor digitorum brevis and the extensor hallucis brevis of the rhesus monkey were autotransplanted in the sacrospinal muscle,and they were biopsied at intervals and stained with HE,Bielshowsky and enzyme.

将恒河猴的双足踇、趾短伸肌8块分2组去神经后游离移植于骶棘肌内，定期活检行光镜(HE染色、银染色、酶染色)及电镜观察肌纤维及神经纤维。

Materials and methods: The lung preparation of 6 SARS death patient (died in 9,14,20,29,33,38d) and 6 macaque model (killed in 7,12,14,14, 32, 35d)were objects. Pathological changes, collagen fibers, lattice fibers, elastic fibers, collagen I and III in lungs and fine structure changes were studied by routine H.E dyeing, trigeminy dyeing, trinitrophenol- sirius red staining and polarization microscope, electron microscope and image analysis. Expression of Vimentin、 TGFβ_1、 TNF α、 IL-1β and MMP-2 were detected by immunohistochemistry.Results:1. Pathological changes of SARS death patient.

材料与方法：以6例SARS死亡患者(分别于发病后9、14、20、29、33、38d死亡)和6只猕猴实验模型(分别于染毒后7、12、14、14、32、35d活杀取材)肺标本为对象，应用H.E染色、三联染色、苦味酸-天狼猩红-偏振光法、电镜和图像分析等技术，对比性观察SARS肺组织病理变化和纤维化的病理过程、胶原纤维、网状纤维和弹力纤维的变化特点、Ⅰ型与Ⅲ型胶原纤维的数量和分布规律，旨在探讨SARS肺纤维化的病变经历过程及特点；利用免疫组织化学和形态计量学技术检测SARS死亡患者肺脏的Vimentin、TGFβ_1、TNFα、IL-1β和MMP-2等与炎症反应和纤维化相关活性因子，探讨其发病机制。

The coverslip with differentiated cells were fixed at different timepoints and used for toludine blue or inmunohistochemical dying Meanwhile, different doses of antibody to Fibronectin were added to observe aggregating phenomenon.

用2，3传代细胞按一定比例种植于24孔板内，加入TGF-β1 1，5，10ng／mL，分别于不同时间点取材固定，用于甲苯胺兰组织学染色及抗纤维连接蛋白抗体免疫组化染色。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。