染色于

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

An IPGphor unit with immobile pH gradient strips was used in and the Ettan DALT Ⅱ system under constant power and temperature in SDS-PAGE. The 2-D gel was stained by both methods of coomassie blue and silver.

第一向采用固定pH值梯度胶条在IPGphor等电聚焦仪上进行等电聚焦，第二向在恒功率且恒温条件下于Ettan-DALTⅡ高通量电泳仪上进行SDS-PAGE电泳，以银染和考马斯亮蓝两种方法染色。

An IPGphorTM unit with immobile pH gradient strips was used in and the EttanTM DALT Ⅱ system under constant power and temperature in SDS-PAGE. The 2-D gel was stained by both methods of coomassie blue and silver.

第一向采用固定pH值梯度胶条在IPGphorTM等电聚焦仪上进行等电聚焦，第二向在恒功率且恒温条件下于Ettan-DALTTMⅡ高通量电泳仪上进行SDS-PAGE电泳，以银染和考马斯亮蓝两种方法染色。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Results: Mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. After ovum implantation ,the mast cells were comparatively less at the place of implantation. The number of mast cells of early and late pregnancy(1～5 dyas, 15～19 dyas) was apparently higher than that during middle pregnancy(P＜0. 05). Stained by alcian blue-safranin, both red and reddish blue mast cells decreased gradually in number during pregnancy, but the proportion of blue mast cells was increased. Critical electrolyte concentration of mast cells decreased as time went on.

结果：(1)肥大细胞主要分布于子宫肌层，子宫内膜较少，且胚泡植人部位的肥大细胞明显少于非植入部位；(2)妊娠早期(孕1～5d)和妊娠后期(孕15～19d)肥大细胞数显著多于妊娠中期(P＜0.05)；(3)Alcian蓝藏红组化染色后，可见妊娠过程中红色和红蓝混合色肥大细胞逐渐减少；蓝色肥大细胞所占比例逐渐增大，孕9～19d肥大细胞几乎全部为蓝色；(4)肥大细胞临界电解质浓度值随妊娠时间的推移而变小。

Dyeable and non-dyeable polypropylene is a new innovative material which is suitable for many special uses due to its multi-functionality.

可染色丙纶纤维是一中革命性新型功能纤维，具有众多独特性能，非常适合于一系列专门用途的功能性面料。

The positive hybridizations with COCH gene cRNA probe were detected in the mouse mesenchymic cell surroundding the endolymphic sac epithelium, the level of expression was similar to the fibrocytes underlying crista ampullaris sense epithellium, and inferior to the fibrocytes of spiral ligament.

成年小鼠内淋巴囊周围间质细胞COCH基因表达阳性，其原位杂交染色平均积分光密度为0.4±0.25（〓±SD，单位：U／mm〓），与壶腹嵴感觉上皮下间质细胞的0.47±0.21相似，弱于耳蜗螺旋韧带成纤维细胞的0.77±0.19。

Results These immunoreactivities of nm23-H1 protein were most common in the cytoplasm of tumor and epithelia cell.

结果 nm23-H1蛋白染色阳性信号主要定位于肿瘤和上皮细胞胞浆中。

It is as soft as nylon and as fluey as acylic.PTT possesses the many advantages of many synthetic fibers and these advantages make its application widly. Currently, the protucts of PTT include carpet, garment, plastic and so on. This paper is about the application of filament CM800 in knitted underwear fabrics. Filament CM800 is bi -component fibers with excellent elastic recovery. According to the performance of knitting fabrics, the relationship of knitting technique and wearablity is researched by orthogonal methods.

与常规化纤相比，PTT纤维具有突出的化学稳定性、回弹性，较好的染色性和色牢度以及良好的抗污性，它综合了锦纶的柔软性、腈纶的蓬松性、将多种纤维的优良性能集于一体，这些优势使它具有广阔的应用空间，目前PTT的产品主要涉及地毯领域、服装领域和工程领域等。

Methods The unfixed tissue blocks were put into the freezing chamber and cut it for 5 or 10 μm section at once after it were frozen,then put it into the fixactives for 20 seconds and stained with haematoxylin and eosin.

将未经固定的组织块放入冷冻箱内，冷冻后切片5μm或10μm，即刻于固定液里固定20s，用HE染色。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。