染色于

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标：于造模后15 d各组取1只大鼠行组织形态学观察；造模后21，30 d，每组各处死8只大鼠，取材后，行苏木精-伊红染色，免疫组织化学检测增殖细胞核抗原的表达，并计算成纤维细胞的增殖指数。

At room temperature before 500 μL of PBS was added to make a unicellular suspension.

流式细胞术（flow cytometry， FCM）以其对荧光染色的单细胞悬液进行快速、准确的测量和分析而广泛应用于细胞生物学、肿瘤学、免疫学等领域，特别是多种识别白细胞抗原的单克隆抗体的出现，使FCM 在外周血白细胞分析的基础研究和临床诊断中得到了广泛的应用［1］。

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

At the early stage of 〓 incubation, the mitochondrias were hyperplasia and swelling. After that the mitochondrias became vacuolation.

砷剂孵育早期即可见线粒体增生和肿胀，线粒体的改变早于细胞核染色质的变化。

Air dried nuclei flatten out (as opposed to well-fixed cells, which maintain a more 3-dimensional shape), and do not take up stain very well. This leads to enlarged pale appearance.

风干使细胞核变平(相对于保存良好的细胞，保持更好的三维立体结构外形)，且不能很好的被染色，风干导致核增大、暗淡。

The influence of gas type, treatment time, and post-treatment dyeing on the wicking and drying properties of the treated fabrics were studied.

对气体种类、处理时间及后处理染色对羊毛织物吸湿快于性能产生的影响进行了研究。

INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.

干预：实验组腹腔注射催吐剂顺氯氨铂10 mg/kg，对照组注射等剂量的生理盐水后，于室温、安静、避光环境下观察大鼠的行为学变化。6 h后取大鼠脑组织，进行冷冻连续切片，用免疫组织化学染色方法观察大鼠脑干和前脑核团的Fos阳性神经元的分布，并进行阳性细胞记数。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Paibao was cstablished over ten years,has strong power advanced techniques,can supply plenty of all range of natural building colour materials,as machine-made cobblestone riverpebble、brocatello、spraying pat stone、landscape karst、 fossile、culture stone、natural colour gravel and dying pebble for decoration indoor,They have non-poison、tasteless and fadeless,also hard enough,colour shining,simple and unsophisticated,has specific property of compressive resistance,wearability,corrosion resistance,are perfect environmental protection building material and videly used in public building、villa、courtyard、garden and high building,spreading andtique eastern culture,reflecting art style of simples and unsophisticated、quiet and tastefully from the western.

我厂是建厂十几年的企业，实力雄厚、技术先进。大量生产供应各种天然颜色的机制鹅卵石、河卵石、雨花石、干粘石、喷刷石、造景石、木化石、文化石、天然色理石米等建筑装饰材料及室内装饰用的高级染色沙，无毒、无味、不脱色。品质坚硬，色泽鲜明古朴，具有高压、耐磨腐蚀的天然特性，是一种理想的绿色建筑材料。被广泛应用于公共建筑、别墅建筑、园林艺术和其它高级上层建筑。它既宏扬东方古老的文化，又体现西方古典、优雅，返朴归真的艺术风格。

Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2,〔53+54=(31.3±24.6)ng/mg vs. S1+S2=(5.7±4.8) ng/mg, P.01〕. Cells immunoreactive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with fibrosis. Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.

慢性肝炎纤维化分期重的S3和S4患者，其肝组织中Chymase浓度〔S3+S4=(31.3±24.6)ng/mg〕明显高于纤维化轻的S1和S2患者〔S1+S2=(5.7±4.8)ng/mg〕，P.01；免疫组化染色结果，Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁，其分布与纤维化部位相一致，且肝组织中Chymase浓度高的患者，其肝组织内Chymase标记的肥大细胞分布增多推测肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐