染色于

The surface antigen expressions of MNC were detected by flow cytometry. MNC were induced with DMEM medium, containing HGF alone, FGF4 alone, HGF+FGF4 or no growth factor, respectively. The markers of hepatic linear cells were examined before induction, and 7, 14, 21 and 28 d after induction by RT-PCR, immunocytochemistry and periodic acid-schiff in every group.

分别用肝细胞生长因子、成纤维细胞生长因子-4(FGF4)、HGF+FGF4、无生长因子4种处理因素进行诱导培养，于诱导前及诱导后的第7、14、21、28天采用RT-PCR检测AFP、白蛋白及C-met、FGFR2 mRNA的表达，免疫细胞化学法检测肝细胞抗原和CK18的表达，PAS 法进行糖原染色。

Results: the positive cells mostly included ependymal cells of myelocoele, glial cells, anterior horn motor neurons, and epithelial cells of spinal pia mater and their expressions were mainly located on cell membrane. interestingly, aqp4 distribution in glial cells and ependymal cells showed certain directivity, but the former was mainly in the membrane side contacting with capillary endothelial cells and the latter mostly in the basolateral membrane of ependymal cells.

结果： 免疫组织化学染色和原位杂交结果显示胶质细胞、前角运动神经元、中央管室管膜细胞、软脊膜上皮细胞均表达阳性，aqp4主要分布于细胞膜，其中，胶质细胞和中央管室管膜细胞表达存在极性，前者主要分布在与毛细血管内皮细胞接触的一侧，后者主要分布在中央管室管膜细胞的基底侧膜。

A corneal abrasion with a diameter of 7 mm was created centrally in both eyes with a trephine and subsequent rubbing off on the corneal epithelium with a 20% alcohol soaked swab. Photographic documentation and hematoxylin and eosin stained pathological section were performed when rabbits were killed at 0,12,24,36,48 and 72 h. Planimetry was performed, and the corneal photographs were analyzed with computer software.

将4组兔双眼角膜中央置入直径7.00mm的环钻，其内滴入20%乙醇约60s后用角膜刮匙刮去角膜上皮，制作角膜上皮缺损模型，术后每组滴用眼液频率均为3次／d，各组分别于术后12，24，36，48及72h行裂隙灯下荧光素染色照相及海德堡共焦激光角膜显微镜检查后各处死2只兔，取角膜做病理切片同时应用计算机图像处理系统测量角膜损伤后愈合面积。

Tibetan Dream Carpets are made in Tibet, adopted traditional technique of handspun yarn and vegetable dying, which have characteristics of naturalness, simplicity, primitiveness and exquisiteness.

藏之梦藏毯产自于西藏，是采用传统的手工纺纱工艺和植物染色编织的地毯，该产品自然、粗犷、古朴、典雅。

METHODS:After the treatment of TRAIL or LPS at different doses,we tested the nuclear translocation of NF-κB by cell immunohistochemical staining and electrophoretic mobility shift assay,and evaluated the level of IκB by RT-PCR under pyrrolidine dithiocarbamatetreatment.

当不同浓度的TRAIL和LPS作用于细胞后，我们通过细胞免疫组化染色和凝胶电泳迁移试验来检测NF-κB核转位的情况。并通过RT-PCR的方法粗略评定二硫代氨基甲酸吡咯烷对抑制蛋白IκB的影响。

BMI-1(B-cell-specific Moloney murine leukemia virus integration site 1), a Polycomb group repressor, is always present as a PcG complex combined with RING1, HPC2, Mph2, et al. BMI-1 prolongs cells'capacity to proliferate and represses their senescence by repressing the transcription of CDKN2A through acetylhydrolasing or deacetylhydrolasing the Polycomb response elements in chromosome.

BMI-1 (B-cell-specific Moloney murine leukemia virus integration site 1)属于PcG家族，它与该家族其他成员如RING1，HPC2，Mph2等结合形成蛋白复合体，作用于染色质PREs位点，通过对组蛋白进行乙酰化和去乙酰化修饰，从而稳定抑制CDKN2A转录，最终促进细胞的增殖并抑制细胞凋亡。

"We were familiar with the control work done by IBM and a steel company in Cleveland, and thought this could be applied to paper processes," says Rutledge. It was used for applying color, coatings, fillers and other additives in the papermaking process.

Rutledge说：我们很熟悉IBM的控制运作，也熟悉一个在Cleveland的钢铁厂，并想象着能适用于造纸过程：用来染色、涂布、填料和其它造纸过程中的活动。

The recovered thalli wereeneymed into single cells by sea snail enzyme.Cell viability was measuredwith the method of Evans Blue and Neutral Red dye.Cells were cultured atthe same condition.

复苏的紫菜经海螺酶解离成为单细胞，通过染色检查其细胞活性并且将单细胞培养于适宜的条件下，观察其生长和发育状况，以了解不同复苏条件对冷藏叶状体细胞生理状态的恢复程度。

Objective To study the staining method by silver nitrate in preparation of teaching-sample sections in quantity.

目的探讨适用于大批制作大脑锥体细胞教学标本切片浸银染色方法。

The second part of the study examined the distribution of type Ⅰ and Ⅲ collagens, fibronectin and laminin in murine liver using protein A gold silver tealnique.

研究的第二部分建立了光镜水平的葡萄球菌A蛋白金银染色法并将其运用于对肝脏基质抗原FN和LN的免疫细胞化学研究，获得了优于免疫荧光法和免疫酶法的结果。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐